Genomic Diversity of Type B3 Bacteriophages of Caulobacter crescentus
Genomic Diversity of Type B3 Bacteriophages of Caulobacter crescentus
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DOI：
10.1007/s00284-017-1248-4
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发表时间：
2017-07-01
影响因子：
2.6
通讯作者：
Ely, Bert
中科院分区：
文献类型：
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作者：
Ash, Kurt T.;Drake, Kristina M.;Ely, Bert
感染新月柄杆菌的 B3 型噬菌体基因组是迄今为止存入 GenBank 的最大噬菌体基因组之一，大小超过 200 kb。在这项研究中，我们引入了六种新的噬菌体基因组，这些基因组是从美国东南部的各个水系统和全球热带地区收集的噬菌体获得的。对 12 个可用基因组的比较分析揭示了一个“核心基因组”，约占这些噬菌体基因组的 1/3，并且主要位于头部、尾部和裂解基因区域。尽管是从不同的地理位置分离出来的，但这些噬菌体的基因组在基因组序列和基因顺序上都高度保守。我们还鉴定了导致这组噬菌体基因组多样性的插入、缺失、易位和水平基因转移事件，并证明这些变化与这组噬菌体中发生基因组模块化重配的观点不一致。
The genomes of the type B3 bacteriophages that infect Caulobacter crescentus are among the largest phage genomes thus far deposited into GenBank with sizes over 200 kb. In this study, we introduce six new bacteriophage genomes which were obtained from phage collected from various water systems in the southeastern United States and from tropical locations across the globe. A comparative analysis of the 12 available genomes revealed a "core genome" which accounts for roughly 1/3 of these bacteriophage genomes and is predominately localized to the head, tail, and lysis gene regions. Despite being isolated from geographically distinct locations, the genomes of these bacteriophages are highly conserved in both genome sequence and gene order. We also identified the insertions, deletions, translocations, and horizontal gene transfer events which are responsible for the genomic diversity of this group of bacteriophages and demonstrated that these changes are not consistent with the idea that modular reassortment of genomes occurs in this group of bacteriophages.