PLASMINOGEN-ACTIVATOR SECRETION BY GRANULE NEURONS IN CULTURES OF DEVELOPING CEREBELLUM
PLASMINOGEN-ACTIVATOR SECRETION BY GRANULE NEURONS IN CULTURES OF DEVELOPING CEREBELLUM
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DOI：
10.1073/pnas.78.12.7810
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
SEEDS, NW
中科院分区：
文献类型：
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作者：
KRYSTOSEK, A;SEEDS, NW
从出生后7-9天的小鼠小脑建立分散的细胞培养物。纤维蛋白玻片法用于获得纤溶酶原激活剂生产的定位到特定的细胞。纤溶活性细胞小（直径5- 8 μ m）、圆并且单个或聚集出现。纤维蛋白溶解是纤溶酶原和时间依赖性的，可被ε-抑制。当细胞在纤维蛋白覆盖之前固定在福尔马林中时，不会出现这种情况。只有当颗粒神经元丰富的文化发生强烈的纤维蛋白降解。这些纤溶酶原激活物分泌细胞被确定为颗粒神经元的细胞分离方法，核形态和它们的能力，结合破伤风毒素和兔抗血清抗小鼠小脑（抗Cbl-1抗血清）。纤溶酶原激活剂可以定量在分级的组织匀浆或在细胞培养基中的125 I标记的纤维蛋白平板测定。小脑提取物中的纤维蛋白溶解是95%依赖于添加纤溶酶原的存在下，更多的，活性是更大的小脑提取物相比，大脑皮层的相同年龄。在所检查的年龄，小脑含有许多迁移神经元，纤溶酶原激活物的产生可能参与细胞运动的过程。
Dispersed cell cultures were established from 7-9 day postnatal mouse cerebellum. The fibrin slide method was used to obtain a localization of plasminogen activator production to specific cells. Fibrinolytically active cells were small (5- to 8-.mu.m diameter), round and occurred singly or in aggregates. Fibrinolysis was both plasminogen and time dependent, inihibitable by .epsilon.-aminocaproic acid and soybean trypsin inhibitor and did not occur when cells were fixed in formalin prior to the fibrin overlay. Strong fibrin degradation occurred only when granule neurons were abundant in the cultures. These plasminogen activator secreting cells were identified as granule neurons by cell separation methods, nuclear morphology and their ability to bind tetanus toxin and rabbit antiserum against mouse cerebellum (anti-Cbl-1 antiserum). Plasminogen activator could be quantified in fractionated tissue homogenates or in cell culture medium by the 125I-labeled fibrin plate assay. Fibrinolysis in cerebellar extracts was 95% dependent on the presence of added plasminogen; more, the activity was greater in cerebellar extracts compared to cerebral cortex of the same age. At the age examined, the cerebellum contains many migratory neurons, and plasminogen activator production may be involved in the process of cell movement.