Dexamethasone suppresses expression of nuclear factor-kappaB in the cells of tracheobronchial lavage fluid in premature neonates with respiratory distress
Dexamethasone suppresses expression of nuclear factor-kappaB in the cells of tracheobronchial lavage fluid in premature neonates with respiratory distress
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DOI：
10.1203/01.pdr.0000219120.92049.b3
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发表时间：
2006-06-01
影响因子：
3.6
通讯作者：
Rahman, Irfan
中科院分区：
文献类型：
--
作者：
Aghai, Zubair H.;Kumar, Sanjay;Rahman, Irfan
核因子-κ B（NF-κ B）在调节急性肺损伤中的关键炎症介质中起核心作用。类固醇通过抑制促炎细胞因子的抗炎作用可能是通过抑制转录因子NF-κ B介导的。本研究旨在探讨糖皮质激素治疗对早产儿呼吸窘迫气管支气管灌洗液（TBLF）细胞NF-κ B B表达的影响。19名需要机械通气并接受糖皮质激素治疗的早产儿入组。他们的胎龄（平均值+/- SD）为25.0 +/- 1.2周，出生体重为714 +/- 105 g，开始使用地塞米松的年龄为33 +/- 15 d。在开始地塞米松治疗前和治疗后48-72 h收集气管支气管灌洗液。使用小鼠MA B针对细胞离心涂片上NF-κ B的p65亚基，通过免疫细胞化学测定NF-κ B表达。与类固醇治疗后相比，开始地塞米松治疗前染色细胞百分比和染色强度指数显着较高。在开始地塞米松治疗后，NF-κ B B在单个核细胞的细胞质和细胞核中的定位显著降低。开始使用地塞米松后，IL-8的浓度也显著降低。结论：地塞米松可抑制呼吸窘迫早产儿TBLF中NF-κ B B在单核细胞胞浆和胞核中的表达，降低IL-8水平。皮质类固醇的抗炎作用可能通过NF-κ B B介导。
Nuclear Factor-kappaB (NF-kappa B) plays a central role in regulating the key mediators of inflammation involved in acute lung injury. The anti-inflammatory effect of steroids by suppressing pro-inflammatory cytokines may be mediated by inhibition of transcription factor NF-kappa B. The objective of this study was to determine the effect of glucocorticoid therapy on the expression of NF-kappa B in the cells of tracheobronchial lavage fluid (TBLF) in premature neonates with respiratory distress. Nineteen premature neonates requiring mechanical ventilation and receiving glucocorticoids were enrolled. Their gestational age (mean +/- SD) was 25.0 +/- 1.2 wk, birth weight 714 +/- 105 g and age of starting dexamethasone was 33 +/- 15 d. Tracheobronchial lavage fluid was collected before and 48-72 h after starting dexamethasone. NF-kappa B expression was measured by immunocytochemistry using mouse MAb against the p65 subunit of NF-kappa B on cytospin slides. The percent of cells stained and the intensity staining index were significantly higher before starting dexamethasone compared with after steroid therapy. Localization of NF-kappa B was significantly decreased in the cytoplasm and nuclei of mononuclear cells after initiation of dexamethasone therapy. The concentration of IL-8 was also significantly lower after starting dexamethasone. In conclusion, dexamethasone suppressed the expression of NF-kappa B in the cytoplasm and nuclei of mononuclear cells and decreased levels of IL-8 in TBLF from premature neonates with respiratory distress. The anti-inflammatory effects of corticosteroids may be mediated through NF-kappa B.