TIMED APPEARANCE OF A CALCIUM-BINDING PROTEIN CONTAINING GAMMA-CARBOXYGLUTAMIC ACID IN DEVELOPING CHICK BONE
TIMED APPEARANCE OF A CALCIUM-BINDING PROTEIN CONTAINING GAMMA-CARBOXYGLUTAMIC ACID IN DEVELOPING CHICK BONE
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DOI：
10.1016/0012-1606(78)90038-6
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发表时间：
1978-01-01
影响因子：
2.7
通讯作者：
REID, ML
中科院分区：
文献类型：
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作者：
HAUSCHKA, PV;REID, ML
骨含有小蛋白质，富含维生素K依赖性Ca结合氨基酸γ。羧基谷氨酸（Gla）。这种蛋白质被命名为骨钙素，可通过中性EDTA脱矿作用提取，并且含有骨中发现的总肽Gla的80%以上。骨钙素以中等亲和力结合Ca 2+（2 mol Ca 2 +/6500 g蛋白质; Kd = 0.83 mM）。骨钙素出现在鸡胚骨（下颌骨、颅骨、胫跗骨和股骨）中，与受精后8-12天第一次组织学观察到的骨矿物质沉积一致。这种蛋白质的量在发育过程中急剧增加，具有每种类型骨的特征性发作和动力学。在长骨骨干（骨干中段）中，以骨钙素为代表的非胶原蛋白的比例在第8 - 20天之间增加100至200倍。相对于总骨蛋白，增加约35倍。骨钙蛋白可能在矿化组织的发育中起作用，并且可能是相对于骨和/或软骨形成分化的细胞的特征性产物。
Bone contains a small protein, rich in the vitamin K-dependent Ca-binding amino acid .gamma.-carboxyglutamate (Gla). This protein, named osteocalcin, is extractable by neutral EDTA demineralization and contains over 80% of the total peptide Gla found in bone. Osteocalcin binds Ca2+ with moderate affinity (2 mol of Ca2+/6500 g of protein; Kd = 0.83 mM). Osteocalcin appears in embryonic chick bones (mandible, calvaria, tibiotarsus and femur) coincident with the 1st histologically observable deposition of bone mineral at 8-12 days after fertilization. The quantity of this protein increa ses dramatically during development with characteristic onset and kinetics for each type of bone. In the long bone diaphysis (midshaft), the fraction of noncollagen protein represented by osteocalcin increases 100 to 200-fold between the 8th-20th day. Relative to total bone protein, the increase is about 35-fold. Osteocalcin may play a role in development of mineralized tissues and may be a characteristic product of cells differentiated with respect to bone and/or cartilage formation.