SECRETION AND CONTRIBUTION TO LIPOLYSIS OF GASTRIC AND PANCREATIC LIPASES DURING A TEST MEAL IN HUMANS
SECRETION AND CONTRIBUTION TO LIPOLYSIS OF GASTRIC AND PANCREATIC LIPASES DURING A TEST MEAL IN HUMANS
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DOI：
10.1016/0016-5085(93)90908-u
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发表时间：
1993-09-01
期刊：
影响因子：
29.4
通讯作者：
LAUGIER, R
中科院分区：
文献类型：
--
作者：
CARRIERE, F;BARROWMAN, JA;LAUGIER, R
背景：本研究的目的是定量评估胃和胰脂肪酶对体内脂肪分解的相对贡献。方法：测量健康志愿者消化液体测试餐期间胃和胰脂肪酶的分泌物，并测定其在十二指肠液中的各自水平。通过使用特异性酶测定和酶联免疫吸附测定，可以清楚地区分胃脂肪酶活性与胰脂肪酶活性。在整个消化过程中对脂肪分解产物进行监测。结果：以重量计，消化3小时后胰脂肪酶与胃脂肪酶总分泌量的比率约为4。胃水解水平计算为膳食甘油三酯释放的酰基链的 10% ± 1%。胃脂肪酶在十二指肠中仍保持活性，仍可水解7.5%的甘油三酯酰基链。结论：在整个消化过程中，胃脂肪酶可水解17.5%的甘油三酯酰基链。换句话说，胃脂肪酶可能会水解4个酰基链中的1个，需要水解该酰基链才能使肠道完全吸收由两个甘油三酯分子降解产生的单酸甘油酯和游离脂肪酸。
Background:The aim of this study was to quantitatively evaluate the relative contributions to in vivo lipolysis of gastric and pancreatic lipases.Methods:Gastric and pancreatic lipase secretions were measured, and their respective levels were determined in duodenal fluid during the digestion of a liquid test meal in healthy volunteers. Gastric lipase activity was clearly distinguished from that of pancreatic lipase by using both a specific enzymatic assay and an enzyme-linked immunosorbent assay. Lipolysis products were monitored throughout the digestion period.Results:On a weight basis, the ratio of pancreatic lipase to gastric lipase total secretory outputs was found to be around four after 3 hours of digestion. The level of gastric hydrolysis was calculated to be 10% ± 1% of the acyl chains released from the meal triglycerides. Gastric lipase remained active in the duodenum where it might still hydrolyze 7.5% of the triglyceride acyl chains.Conclusions:Globally during the whole digestion period, gastric lipase might hydrolyze 17.5% of the triglyceride acyl chains. In other words, gastric lipase might hydrolyze 1 acyl chain of 4, which need to be hydrolyzed for a complete intestinal absorption of monoglycerides and free fatty acids resulting from the degradation of two triglyceride molecules.