Triterpene saponins from Vietnamese ginseng (Panax vietnamensis) and their hepatocytoprotective activity
Triterpene saponins from Vietnamese ginseng (Panax vietnamensis) and their hepatocytoprotective activity
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DOI：
10.1021/np000393f
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发表时间：
2001-04-01
影响因子：
5.1
通讯作者：
Kadota, S
中科院分区：
文献类型：
--
作者：
Le Tran, Q;Adnyana, IK;Kadota, S
越南人参（Panax vietnamensis）的甲醇提取物被发现对原代培养的小鼠肝细胞中 D-半乳糖胺（D-GalN）/肿瘤坏死因子-α（TNF-α）诱导的细胞死亡具有肝细胞保护作用。对提取物进行进一步的化学研究，得到两种新的达玛烷型三萜皂苷，人参皂苷 Rh-5 (1) 和长春人参皂苷 R-25 (2)，以及八种已知的达玛烷型三萜皂苷，majonoside R-2 (3)、假人参皂苷 RT4 (4)、长春人参皂苷 R-1 (5)、R-2 (6) 和R-10 (7)、人参皂苷 Rg(1) (8)、Rh-1 (9) 和 Rh-4 (10)，以及已知的皂苷元原人参三醇氧化物 II (11)。在光谱分析的基础上阐明了它们的结构。此外，通过使用LC-电喷雾电离(ESI)-MS方法，还在提取物中鉴定出五种已知的皂苷：人参皂苷Rb-1、Rb-2、Re、Rd和Re(12-16)。在分离的化合物中，majonoside R-2 (3)（越南人参中的主要皂苷）对原代培养的小鼠肝细胞中 D-GalN/TNF-α 诱导的细胞死亡表现出强大的保护活性。这表明越南人参的肝细胞保护作用是由于具有奥克替洛尔型侧链的达玛烷型三萜皂苷。越南人参的特征成分。
The methanol extract of Vietnamese ginseng (Panax vietnamensis) was found to possess hepatocytoprotective effects on D-galactosamine (D-GalN)/tumor necrosis factor-alpha (TNF-alpha)-induced cell death in primary cultured mouse hepatocytes. Further chemical investigation of the extract afforded two new dammarane-type triterpene saponins, ginsenoside Rh-5 (1) and vina-ginsenoside R-25 (2), as well as eight known dammarane-type triterpene saponins, majonoside R-2 (3), pseudo-ginsenoside RT4 (4), vina-ginsenosides R-1 (5), R-2 (6), and R-10 (7), ginsenosides Rg(1) (8), Rh-1 (9), and Rh-4 (10), and a known sapogenin protopanaxatriol oxide II (11). Their structures were elucidated on the basis of spectral analysis. In addition, by the using LC-electrospray ionization (ESI)-MS method, five known saponins, ginsenosides Rb-1, Rb-2, Re, Rd, and Re (12-16), were also identified in the extract. Among the compounds isolated, majonoside R-2 (3), the main saponin in Vietnamese ginseng, showed strong protective activity against D-GalN/TNF-alpha -induced cell death in primary cultured mouse hepatocytes. This demonstrates that the hepatocytoprotective effect of Vietnamese ginseng is due to dammarane-type triterpene saponins that have an ocotillol-type side chain. a characteristic constituent of Vietnamese ginseng.