Factors Influencing the Deaggregation of Human and Rabbit Platelets
Factors Influencing the Deaggregation of Human and Rabbit Platelets
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影响人和兔血小板解聚的因素
DOI：
10.1055/s-0038-1657353
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发表时间：
1983
影响因子：
6.7
通讯作者：
E. Harfenist
中科院分区：
文献类型：
--
作者：
R. Kinlough;J. Mustard;D. Perry;Elisabetta Dejana;Cazenave Jp;Packham Ma;E. Harfenist
摘要 血小板解聚的机制尚不清楚。如果没有发生释放反应，可以用 EDTA 或 PGI2 解聚由 ADP 聚集的兔或人的洗涤血小板；在解聚过程中，125I-纤维蛋白原从血小板上解离出来。悬浮在不含钙的介质中的人血小板在 ADP 诱导的聚集过程中发生释放反应； EDTA、PGE 或 PGI2 不会解聚这些血小板，尽管 EDTA 会取代聚集过程中与其结合的大部分 125I-纤维蛋白原。通过低浓度的释放诱导刺激（花生四烯酸钠、胶原蛋白或凝血酶）聚集的兔血小板可以通过 EDTA、PGI2 或 PGE1 解聚，并且 125I-纤维蛋白原从中解离；当胶原蛋白或凝血酶浓度较高时，l25I-纤维蛋白原的解聚和解离速度较慢。因凝血酶、胶原蛋白或花生四烯酸钠和 ADP 的组合而发生释放反应的人血小板不容易被 EDTA 或 PGE1 解聚。由于涉及糖蛋白 IIb/IIIa 复合物的聚集和纤维蛋白原结合很容易被 EDTA 逆转，并且由于血小板反应蛋白与活化血小板结合需要 Ca2+，因此可能存在不受 EDTA 破坏的第三种类型的血小板-血小板粘附；这种类型的结合对人血小板的作用比对兔血小板的作用更大。
Summary The mechanisms involved in platelet deaggregation are unclear. Washed platelets from rabbits or humans aggregated by ADP can be deaggregated by EDTA or PGI2 if the release reaction has not occurred; during deaggregation 125I-fibrinogen dissociates from the platelets. Human platelets suspended in a medium without calcium undergo the release reaction during ADP-induced aggregation; EDTA, PGE, or PGI2 do not deaggregate these platelets although EDTA displaces much of the 125I-fibrinogen that associates with them during aggregation. Rabbit platelets aggregated by low concentrations of releaseinducing stimuli (sodium arachidonate, collagen or thrombin) can be deaggregated by EDTA, PGI2 or PGE1 and 125I-fibrinogen dissociates from them; with high concentrations of collagen or thrombin, deaggregation and dissociation of l25I-fibrinogen is slower. Human platelets that have undergone the release reaction in response to thrombin, collagen or a combination of sodium arachidonate and ADP are not readily deaggregated by EDTA or PGE1. Since aggregation and fibrinogen binding involving the glycoprotein IIb/IIIa complex are readily reversed by EDTA, and since Ca2+ is required for thrombospondin binding to activated platelets, there may be a third type of platelet-platelet adherence that is not disrupted by EDTA; this type of binding plays a greater role with human than with rabbit platelets.
通过蛋白水解酶暴露人血小板上的纤维蛋白原受体。
DOI：
--
发表时间：
1981
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Niewiarowski,S;Budzynski,AZ;Morinelli,TA;Brudzynski,TM;Stewart,GJ
通讯作者：
Stewart,GJ