Purified Adult Ensheathing Glia Fail to Myelinate Axons under Culture Conditions that Enable Schwann Cells to Form Myelin
Purified Adult Ensheathing Glia Fail to Myelinate Axons under Culture Conditions that Enable Schwann Cells to Form Myelin
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DOI：
10.1523/jneurosci.22-14-06083.2002
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发表时间：
2002-07
期刊：
影响因子：
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通讯作者：
G. Plant;P. Currier;Ernesto P Cuervo;Margaret L. Bates;Yelena Pressman;M. Bunge;P. Wood
中科院分区：
文献类型：
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作者：
G. Plant;P. Currier;Ernesto P Cuervo;Margaret L. Bates;Yelena Pressman;M. Bunge;P. Wood
一些研究表明嗅鞘胶质细胞（EG）可以形成雪旺细胞（SC）样髓鞘。由于可能的误解归因于污染SC，EG产生髓鞘的能力需要进一步探索。因此，我们比较了成人EG，纯化的免疫淘洗与p75抗体，和成人SC产生髓鞘时，与纯化的背根神经节神经元（DRGNs）在无血清和含血清培养基共培养的能力。在两种培养基配方中，SC/DRGN培养物中髓鞘的数量远高于EG/DRGN培养物; EG/DRGN培养物中鞘的数量等于未添加细胞的纯化DRGN培养物中的鞘的数量。后者的结果表明，髓鞘形成的少数SC保留在纯化的DRGN培养物可能会发生，表明在EG/DRGN培养物中的髓鞘可能是SC髓鞘。EG和SC过程的轴突的关系显着的差异进行了观察。而SC显示相对较短，厚的过程，吞没轴突在小束或在个别的细胞质沟和隔离较大的轴突成一对一的关系，EG扩展扁平片，分区或仅部分包围的轴突束，有时跨越整个文化。SC表现出典型的外周神经中的SC的行为，而EG表现出类似于EG的嗅神经中的特征。总之，来自成年动物的p75选择的EG没有表现出与轴突的SC样关系，并且没有形成髓鞘。
Several studies have suggested that olfactory ensheathing glia (EG) can form Schwann cell (SC)-like myelin. Because of possible misinterpretation attributable to contaminating SCs, the capacity of EG to produce myelin needs to be explored further. Therefore, we compared the abilities of adult EG, purified by immunopanning with p75 antibody, and adult SCs to produce myelin when cocultured with purified dorsal root ganglion neurons (DRGNs) in serum-free and serum-containing media. In both media formulations, the number of myelin sheaths in SC/DRGN cultures was far higher than in EG/DRGN cultures; the number of sheaths in EG/DRGN cultures was equal to that in purified DRGN cultures without added cells. The latter result demonstrates that myelination by a few SCs remaining in purified DRGN cultures may occur, suggesting that myelin in EG/DRGN cultures could be SC myelin. Striking differences in the relationship of EG and SC processes to axons were observed. Whereas SCs displayed relatively short, thick processes that engulfed axons in small bundles or in individual cytoplasmic furrows and segregated larger axons into one-to-one relationships, EG extended flattened sheets that partitioned or only partially encircled fascicles of axons, sometimes spanning the entire culture. SCs exhibited behavior typical of SCs in peripheral nerves, whereas EG exhibited characteristics resembling those of EG in olfactory nerves. In sum, p75-selected EG from adult animals did not exhibit an SC-like relationship to axons and did not form myelin.