Formation Kinetics and Structural Features of Beta-Amyloid Aggregates by Sedimented Solute NMR
Formation Kinetics and Structural Features of Beta-Amyloid Aggregates by Sedimented Solute NMR
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DOI：
10.1002/cbic.201300141
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发表时间：
2013-09-23
期刊：
影响因子：
3.2
通讯作者：
Ravera, Enrico
中科院分区：
文献类型：
--
作者：
Bertini, Ivano;Gallo, Gianluca;Ravera, Enrico
可溶性有毒β-淀粉样蛋白(A-β)聚集物的积累是这种多肽在阿尔茨海默病病理中所起作用的一个有吸引力的假说。我们引入了超速离心法，无论是魔角旋转(原位)还是制备性超速离心法(异地)，以固定化生物分子并使其适用于固体核磁共振研究(Sed核磁共振)。本文使用原位Sed核磁共振技术，通过同时监测单体的消失和低聚物的出现，来研究可溶性Aβ组件的形成动力学。非原位Sed核磁共振允许我们选择不同的低聚物种，并揭示可溶性Aβ组件的原子水平结构特征。
The accumulation of soluble toxic beta-amyloid (A beta) aggregates is an attractive hypothesis for the role of this peptide in the pathology of Alzheimer's disease. We have introduced sedimentation through ultracentrifugation, either by magic angle spinning (in situ) or preparative ultracentrifuge (ex situ), to immobilize biomolecules and make them amenable for solid-state NMR studies (SedNMR). In situ SedNMR is used here to address the kinetics of formation of soluble A beta assemblies by monitoring the disappearance of the monomer and the appearance of the oligomers simultaneously. Ex situ SedNMR allows us to select different oligomeric species and to reveal atomic-level structural features of soluble A beta assemblies.