A chemical NJ15 affects hypocotyl elongation and shoot gravitropism via cutin polymer formation.
A chemical NJ15 affects hypocotyl elongation and shoot gravitropism via cutin polymer formation.
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化学物质 NJ15 通过角质聚合物的形成影响下胚轴伸长和芽向地性。
DOI：
10.1080/09168451.2018.1484278
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
M. Nakajima and T. Asami
中科院分区：
文献类型：
--
作者：
N. Jaroensanti-Tanaka;S. Miyazaki;A. Hosoi;K. Tanaka;S. Ito;S. Iuchi;T. Nakano;M. Kobayashi;M. Nakajima and T. Asami
我们以前发现了一种化学物质，命名为NJ 15，它抑制生长素和油菜素类固醇反应在黑暗生长拟南芥。为了研究其作用方式，我们在拟南芥突变体库中进行了NJ 15低敏感株系的表型筛选。一个株系（f127）在下胚轴伸长和芽向重力性方面表现出明显的NJ 15-低敏感性。经过进一步的测试，确定了DCR，一种参与角质聚合的酶，在突变体中失去了功能，这导致了它对NJ 15的低敏感性。脂肪酸是角质和表皮蜡等聚合物的基础材料。我们证实了NJ 15影响脂肪酸的生物合成，并且它与已知的表皮蜡形成抑制剂咖啡雌酚不同。基于这些结果，我们提出NJ 15的靶点可能位于角质聚合物形成途径中。缩写：Caf：cafenstrole; DEG：差异表达基因; FDR：错误发现率; FOX：全长cDNA-过表达基因; VLCFA：极长链脂肪酸
We previously found a chemical, designated as NJ15, which inhibited both auxin and brassinosteroid responses in dark-grownArabidopsis. To study its mode of action, we performed a phenotypic screening of NJ15-low-sensitive lines among mutant pools ofArabidopsis. One line (f127) showed clear NJ15-low-sensitivity in terms of hypocotyl elongation and shoot gravitropism. After further testing, it was determined that DCR, an enzyme involved in cutin polymerization, had lost its function in the mutant, which caused its low sensitivity to NJ15. Fatty acids are the base materials for polymers such as cutin and cuticular wax. We confirmed that NJ15 affects fatty acid biosynthesis, and that it does differently from cafenstrole, a known inhibitor of cuticular wax formation. Based on these results, we propose that the target of NJ15 is likely located within the cutin polymer formation pathway.Abbreviations: Caf: cafenstrole; DEG: differentially expressed gene; FDR: false discovery rate; FOX: full length cDNA-overexpressor; VLCFA: very-long-chain fatty acid