Extracellular matrices in peritendinous connective tissue after surgical injury to the chicken flexor tendon
Extracellular matrices in peritendinous connective tissue after surgical injury to the chicken flexor tendon
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DOI：
10.1007/bf00573442
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发表时间：
1996-03-01
影响因子：
2.3
通讯作者：
Hirano, H
中科院分区：
文献类型：
--
作者：
Hiranuma, K;Suzuki, K;Hirano, H
采用狭缝印迹杂交和免疫组织化学方法，研究屈肌腱损伤后纤连蛋白、I 型胶原和 III 型胶原的基因表达以及腱周结缔组织中纤连蛋白的存在，使用手术刀部分撕裂诱导鸡屈肌腱周围的纤维粘连。通过结合硫氰酸胍程序和等电沉淀，从肌腱周围的腱周结缔组织中纯化总 RNA。狭缝印迹杂交显示，肌腱损伤后纤连蛋白以及I型和III型胶原的基因表达逐渐增加，并且在整个16周的实验期间保持较高水平。间接免疫荧光显示纤连蛋白优先位于受伤肌腱周围的腱周结缔组织中。腱周结缔组织中纤连蛋白的长期增加可能与屈肌腱损伤后组织学粘连的发展有关。
Gene expression of fibronectin, type I collagen and type III collagen and the presence of fibronectin in peritendinous connective tissue were investigated, following flexor tendon injury, using slot-blot hybridization and immunohistochemistry, Fibrous adhesion around the chicken flexor tendon was induced by a partial laceration using a surgical knife. Total RNA was purified from the peritendinous connective tissue surrounding a tendon by combining the guanidine thiocyanate procedure and isoelectric precipitation. Slot-blot hybridization revealed that gene expression of fibronectin and collagens type I and type III increased gradually after tendon injury, and levels were maintained high throughout the experimental period of 16 weeks. Indirect immunofluorescence revealed fibronectin to be preferentially located in peritendinous connective tissue around the injured tendon. The prolonged increase of fibronectin in the peritendinous connective tissue may relate to the development of histological adhesion after flexor tendon injury.