BAM 1 and RECEPTOR-LIKE PROTEIN KINASE 2 constitute a signaling pathway and modulate CLE peptide-triggered growth inhibition in Arabidopsis root.
BAM 1 and RECEPTOR-LIKE PROTEIN KINASE 2 constitute a signaling pathway and modulate CLE peptide-triggered growth inhibition in Arabidopsis root.
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DOI：
10.1111/nph.13520
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发表时间：
2015-12
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影响因子：
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通讯作者：
Noriko Shimizu;Takashi Ishida;M. Yamada;S. Shigenobu;Ryo Tabata;Atsuko Kinoshita;K. Yamaguchi;M. Hasebe;K. Mitsumasu;S. Sawa
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作者：
Noriko Shimizu;Takashi Ishida;M. Yamada;S. Shigenobu;Ryo Tabata;Atsuko Kinoshita;K. Yamaguchi;M. Hasebe;K. Mitsumasu;S. Sawa
基于配体受体的信号传导是多细胞生物中协调发育和生理过程的细胞间通讯的手段。在植物中，产生细胞的分生组织利用这种信号来调节其活动并确保正常发育。芽和根系统共享进行这一过程的共同要求;然而，其分子基础在很大程度上是不清楚的。已经表明，在拟南芥中，合成的CLV 3/胚周围区（CLE）肽通过CLAVATA 2（CLV 2）和受体样蛋白激酶2（RPK 2）途径的作用收缩根分生组织。我们对根部抗CLE肽信号传导的突变的遗传筛选确定了BAM 1，它是富含亮氨酸的重复序列受体样激酶（LRR-RLK）家族的成员，也参与了这一途径。BAM 1优先表达于根尖，包括静止中心及其周围的干细胞。我们的遗传分析表明，BAM 1与RPK 2一起发挥作用。使用免疫共沉淀试验，我们表明，BAM 1能够与RPK 2形成异聚体复合物。这些研究结果表明，BAM 1和RPK 2受体构成了一个信号通路，调节细胞增殖的根分生组织和相关分子中采用的根和芽分生组织。
Ligand receptor-based signaling is a means of cell-to-cell communication for coordinating developmental and physiological processes in multicellular organisms. In plants, cell-producing meristems utilize this signaling to regulate their activities and ensure for proper development. Shoot and root systems share common requirements for carrying out this process; however, its molecular basis is largely unclear. It has been suggested that synthetic CLV3/EMBRYO SURROUNDING REGION (CLE) peptide shrinks the root meristem through the actions of CLAVATA2 (CLV2) and the RECEPTOR-LIKE PROTEIN KINASE 2 (RPK2) pathway in Arabidopsis thaliana. Our genetic screening for mutations that resist CLE peptide signaling in roots determined that BAM1, which is a member of the leucine-rich repeat receptor-like kinase (LRR-RLK) family, is also involved in this pathway. BAM1 is preferentially expressed in the root tip, including the quiescent center and its surrounding stem cells. Our genetic analysis revealed that BAM1 functions together with RPK2. Using coimmunoprecipitation assay, we showed that BAM1 is capable of forming heteromeric complexes with RPK2. These findings suggest that the BAM1 and RPK2 receptors constitute a signaling pathway that modulates cell proliferation in the root meristem and that related molecules are employed in root and shoot meristems.