Targeted optical imaging of cancer cells using lectin-binding BODIPY conjugated avidin
Targeted optical imaging of cancer cells using lectin-binding BODIPY conjugated avidin
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DOI：
10.1016/j.bbrc.2006.07.169
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发表时间：
2006-09-29
影响因子：
3.1
通讯作者：
Kobayashi, Hisataka
中科院分区：
文献类型：
--
作者：
Hama, Yukihiro;Urano, Yasuteru;Kobayashi, Hisataka
凝集素（asialo受体家族）在许多发生腹膜转移的肿瘤中表达。为了证明基于凝集素结合的荧光成像适用于多种肿瘤，我们将BODIPY与亲和素（avidin-BODIPY）偶联，利用小鼠腹膜癌模型，在体外研究了9种癌细胞系和体内1种卵巢癌细胞系的肿瘤靶向效果。荧光显微镜和流式细胞术观察，9株细胞系均可见特异性的亲和素- bodipy在细胞内聚集。体内光谱分子成像用avidin-BODIPY清晰地显示腹膜肿瘤病灶，而去糖基化的avidin-BODIPY （neutravidin-BODIPY）仅显示肿瘤病灶的微弱荧光，并伴有较高的背景信号。这些结果表明，使用靶向绿色荧光探针的凝集素靶向分子成像技术在具有腹膜间隙播散倾向的各种癌症中具有潜在的用途。(c) 2006爱思唯尔公司版权所有。
Lectins (asialo-receptor family) are expressed on a number of tumors that develop peritoneal metastases. To demonstrate that fluorescence imaging based on lectin binding is applicable for a variety of tumors, we conjugated BODIPY to avidin (avidin-BODIPY), and studied the efficacy of tumor targeting in 9 cancer cell lines in vitro and an ovarian cancer cell line in vivo using a murine peritoneal cancer model. All 9 cell lines showed specific intracellular accumulation with avidin-BODIPY on fluorescence microscopy and flow cytometry. In vivo spectral molecular imaging clearly visualized the peritoneal tumor foci with avidin-BODIPY, whereas, deglycosylated avidin-BODIPY (neutravidin-BODIPY) showed only minimal fluorescence from the tumor foci and was accompanied by higher background signals. These results suggest the lectin-targeted molecular imaging technique using a targeted green fluorescence probe is potentially useful in a wide variety of cancers with a proclivity for dissemination in the peritoneal space. (c) 2006 Elsevier Inc. All rights reserved.