New LC-MS/MS method with single-step pretreatment analyzes fat-soluble vitamins in plasma and amniotic fluid
New LC-MS/MS method with single-step pretreatment analyzes fat-soluble vitamins in plasma and amniotic fluid
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采用一步预处理的新型 LC-MS/MS 方法分析血浆和羊水中的脂溶性维生素
DOI：
10.1194/jlr.d087569
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发表时间：
2018-09-01
影响因子：
6.5
通讯作者：
Li, Yan
中科院分区：
文献类型：
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作者：
Le, Juan;Yuan, Teng-Fei;Li, Yan
脂溶维生素(FSV)，A，D和E，是产前维生素护理的组成部分。此前，在分子水平上解释孕妇补充FSV如何影响怀孕期间胎儿的证据有限。我们建立了一种简化的LC-MS/MS方法来同时检测母体血浆(MP)和羊水(AF)中的FSV；我们使用这种方法来研究这两种基质中FSV水平之间的相关性。用这种方法，我们避开了常用的液-液萃取或固相萃取方法，取而代之的是用乙腈简单地沉淀蛋白质来制备样品。该方法具有良好的线性、日内和日间不精密度和准确度。并与标准参考物质972a和968f进行了一致性验证。在对50例孕中期MP和AF孕妇的分析中，AF组的视黄醇、25-羟基维生素D3[25(OH)D3]和-生育酚(分别反映维生素A、D和E)的浓度低于MP组。在25(OH)D3(r=0.667；P&t；0.001)和视黄醇(r=0.393；P=0.005)中，MP与AF呈显著正相关，而与生育酚无相关性(r=0.145，P>；0.05)。这种新的LC-MS/MS方法在FSV检测中表现出显著的适用性，所观察到的相关性有助于胎儿发育的研究。
Fat-soluble vitamins (FSVs), A, D, and E, are components of prenatal vitamin care. Previously, limited evidence existed to explain on a molecular level how maternal FSV supplementation affects the fetus during pregnancy. We developed a simplified LC-MS/MS method to simultaneously detect FSVs in maternal plasma (MP) and amniotic fluid (AF); we used this approach to investigate the correlation between FSV levels in these two matrices. With this method, we circumvented frequently used liquid-liquid extraction or solid-phase extraction methods and, instead, used simple protein precipitation with acetonitrile for sample preparation. This method displayed satisfactory linearity, intra- and inter-day imprecision, and accuracy. We validated the consistency with standard reference material 972a and 968f certification. In analysis of MP and AF samples from 50 pregnant women in the second trimester, concentrations of retinol, 25-hydroxyvitamin D3 [25(OH)D3], and -tocopherol (reflecting vitamins A, D, and E, respectively) were lower in AF than in MP. Significant positive correlations existed between MP and AF for 25(OH)D3 (r = 0.667; P < 0.001) and retinol (r = 0.393; P = 0.005), but not for -tocopherol (r = 0.145, P > 0.05). This novel LC-MS/MS method shows prominent applicability for FSV detection and the observed correlations contribute to research on fetal development.