Sequencing and characterization of six cDNAs putatively encoding three pairs of pheromone receptors in two sibling species, Helicoverpa armigera and Helicoverpa assulta.
Sequencing and characterization of six cDNAs putatively encoding three pairs of pheromone receptors in two sibling species, Helicoverpa armigera and Helicoverpa assulta.
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DOI：
10.1016/j.jinsphys.2009.12.002
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发表时间：
2010-06
影响因子：
2.2
通讯作者：
Dan-Dan Zhang-Dan;K. Zhu;Chenzhu Wang
中科院分区：
文献类型：
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作者：
Dan-Dan Zhang-Dan;K. Zhu;Chenzhu Wang
鳞翅目昆虫雄性触角上的信息素受体介导对性信息素成分的特异性和敏感性检测。在这项研究中，我们确定并测序了6个推定的cDNA编码PR从同胞物种，即HarmOR 1，HarmOR 2和HarmOR 3棉铃虫和HassOR 1，HassOR 2和HassOR 3烟夜蛾，这似乎是直向同源物烟夜蛾推定PR基因HvOR 13，HvOR 11和HvOR 16。通过实时定量PCR（qRT-PCR）评价6种PR基因的表达模式。除HarmOR 2和HassOR 2在雌雄触角中表达水平相似外，所有推测的PR基因在成虫触角中均表现出雄性偏好的表达模式。HarmOR 1在雄触角中的表达量显著高于HarmOR 2和HarmOR 3。棉铃虫雄触角中相应的3个PR基因，而棉铃虫雄触角中相应的3个PR基因，assulta显示相似的表达水平。这意味着由HarmOR 1编码的PR与Z11-16：Ald相互作用的作用。HarmOR 1转录水平显著高于HassOR 1。这一结果与性信息素混合物中Z11-16：Ald的比例以及雄成虫触角上Z11-16：Ald感受器的丰度一致。
Pheromone receptors (PRs) on male antennae mediate specific and sensitive detection of sex pheromone components in lepidopterans. In this study, we identified and sequenced six putative cDNAs encoding PRs from sibling species, namely HarmOR1, HarmOR2 and HarmOR3 in Helicoverpa armigera and HassOR1, HassOR2 and HassOR3 in Helicoverpa assulta, which appeared to be orthologs of Heliothis virescens putative PR genes HvOR13, HvOR11 and HvOR16, respectively. Expression patterns of the six PR genes were evaluated by quantitative real-time PCR (qRT-PCR). All the putative PR genes exhibited male-biased expression patterns in adult antennae except for HarmOR2 and HassOR2 that showed similar expression levels in male and female antennae. Expression level of HarmOR1 was significantly higher than those of HarmOR2 and HarmOR3 in male antennae of H. armigera, but the three corresponding PR genes in male antennae of H. assulta showed similar expression levels. This implies the role of the PR encoded by HarmOR1 for interacting with Z11-16:Ald. The level of HarmOR1 transcript was significantly higher than that of HassOR1. These results were consistent with the ratio of Z11-16:Ald in their sex pheromone blends and the abundance of sensilla tuned to Z11-16:Ald on antennae of male adults of the two species.