Trimethoxy Silyl End-Capped Hyperbranched Polyglycidol/Polycaprolactone Particle Gels for Cell Delivery and Tissue Repair: Mechanical Properties, Biocompatibility, and Biodegradability Studies
Trimethoxy Silyl End-Capped Hyperbranched Polyglycidol/Polycaprolactone Particle Gels for Cell Delivery and Tissue Repair: Mechanical Properties, Biocompatibility, and Biodegradability Studies
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DOI：
10.3390/jcs7110451
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发表时间：
2023-10
影响因子：
3.3
通讯作者：
Clara González-Chomón;Vasil M. Garamus;Judith Hoyland;S. Halacheva
中科院分区：
文献类型：
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作者：
Clara González-Chomón;Vasil M. Garamus;Judith Hoyland;S. Halacheva
本研究重点开发基于 PCL-HBPG/1SiHBPG 三嵌段共聚物的新型生物相容性和可生物降解颗粒凝胶支架，该三嵌段共聚物由聚己内酯 (PCL) 核心和两个三甲氧基硅烷基封端超支化聚缩水甘油 (HBPG/1SiHBPG) 外部嵌段组成，具有用于软组织再生的潜力。首次研究了凝胶的组成、结构、力学性能和性能之间的关系，并对共聚物设计参数进行了优化。颗粒凝胶支架是由疏水性最强的 PCL-45HBPG/1SiHBPG 在低温下的浓缩分散体形成的，是 HBPG/1SiHBPG 部分形成的大量氢键以及硅氧烷交联（即 Si-O-Si 键）形成的结果。这些凝胶是在生理温度范围内形成的。在没有紫外线辐射的情况下，物理交联的 PCL-45HBPG/1SiHBPG 颗粒有效且安全地形成了机械强度逐渐增加的凝胶。它们具有高弹性并可通过酶触发分解。该凝胶具有生物相容性，并且有可能在没有外源生物刺激的情况下引起细胞附着和分化。这项研究的成功结果将是目前尚不可用的组织再生新方法的前景。
This study focuses on the development of new biocompatible and biodegradable particle gel scaffolds based on PCL-HBPG/1SiHBPG triblock copolymers composed of a polycaprolactone (PCL) core and two outer blocks of trimethoxysilyl end-capped hyperbranched polyglycidol (HBPG/1SiHBPG) that have the potential to be used in soft tissue regeneration. The relationship between the gel’s composition, structure, mechanical properties, and performance has been investigated for the first time and the copolymer design parameters have been optimized. The particle gel scaffolds were formed from the concentrated dispersions of the most hydrophobic PCL-45HBPG/1SiHBPG at low temperatures, and were the result of the numerous hydrogen bonds formed from the HBPG/1SiHBPG moieties as well as the formation of siloxane crosslinks (i.e., Si–O–Si bonds). These gels were formed in the physiological temperature range. Gels with a mechanical strength that gradually increases were formed from the physically crosslinked PCL-45HBPG/1SiHBPG particles effectively and safely, in the absence of UV radiation. They feature high elasticity and undergo enzyme-triggered disassembly. The gels are biocompatible and have the potential to invoke cell attachment and differentiation in the absence of exogenous biological stimuli. A successful outcome of this study will be the prospect of a new approach for tissue regeneration that is currently not available.