Identification of symbiotically expressed coral mRNAs using a model infection system
Identification of symbiotically expressed coral mRNAs using a model infection system
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DOI：
10.1016/j.bbrc.2005.08.174
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发表时间：
2005-10-28
影响因子：
3.1
通讯作者：
Watanabe, T
中科院分区：
文献类型：
--
作者：
Yuyama, I;Hayakawa, H;Watanabe, T
造礁珊瑚（或造礁珊瑚）与共生的腰鞭毛虫共生藻（Symbiodinium spp.）（通常称为zooxanthropium）。为了建立一个由珊瑚宿主和虫黄藻单克隆种群组成的模式共生系统，对5种共生藻细胞系进行了感染性试验。一个进化枝A3菌株（PL-TS-1）以高密度感染幼虫并促进宿主生长。为了确定参与建立或维持共生的宿主基因，使用抑制性消减杂交技术比较了非共生和PL-TS-1感染的幼年息肉之间的mRNA表达模式。由此鉴定出两种mRNA，其表达水平因共生体的存在而增加两倍以上。其中一个mRNA AtSym-02编码一种新的322个氨基酸的蛋白质，该蛋白质被预测为糖基化的跨膜蛋白。(c)2005年爱思唯尔公司All rights reserved.
Hermatypic (or reef-building) corals live in obligatory Mutualistic symbiosis with the symbiotic dinoflagellates Symbiodinium spp. (generally known as zooxanthellae). In an attempt to establish a model symbiosis system consisting of a coral host and a monoclonal population of zooxanthellae, infectivity of five cultured Symbiodinium cell lines was tested on naturally aposymbiotic juveniles of Acropora tenuis. A clade A3 strain (PL-TS-1) infected the juveniles at high density and promoted growth of the host. To identify host genes involved in the establishment or maintenance of symbiosis, mRNA expression patterns were compared between aposymbiotic and PL-TS-1-infected juvenile polyps using the suppression subtractive hybridization technique. Two mRNAs, the expression levels of which were augmented more than twofold by the presence of the symbionts, were thereby identified. One of the mRNAs, AtSym-02, encodes a novel protein of 322 amino acids which is predicted to be a glycosylated trans-membrane protein. (c) 2005 Elsevier Inc. All rights reserved.