Genetic mapping of mouse heat shock protein genes Hsc4a to chromosome 11 and Hsc74 to chromosome 18 and two Hsc74 pseudogenes to chromosomes X and 8.
Genetic mapping of mouse heat shock protein genes Hsc4a to chromosome 11 and Hsc74 to chromosome 18 and two Hsc74 pseudogenes to chromosomes X and 8.
复制标题
小鼠热休克蛋白基因 Hsc4a 至 11 号染色体、Hsc74 至 18 号染色体以及两个 Hsc74 假基因至 X 和 8 号染色体的遗传图谱。
DOI：
10.1006/geno.1998.5716
复制
发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Kozak,CA
中科院分区：
文献类型：
--
作者：
Hunt,CR;Parsian,AJ;Kozak,CA
结果：利用体细胞杂交技术将小鼠Fubp基因定位在3号染色体上。通过对杰克逊BSS 2回交板进行分型，进一步确定了其图谱位置，发现Fubp在分析的所有94只动物中与Ddit 1共分离（图1B）。因此，将Fubp基因置于小鼠3号染色体的连锁图上，在小鼠3号染色体的远端，距着丝粒70.5cM处（图1A）。信息学. Jax. org/locus. html）对Fubp基因座附近的小鼠突变体的分析显示Va，varitint-waddler。Va是半显性突变。杂合Va小鼠是聋的，并表现出盘旋行为、摇头和多动症。它们的皮毛上有正常颜色的、淡黄色的和白色的斑纹。杂合子的存活率几乎是正常的，而纯合子的死亡率非常高，并且很少有幸存者是可生育的（1，2）。Fubp的定位及其作为核酸结合蛋白和遗传调节因子的功能使其成为该突变体的潜在候选基因。同源性：与人类染色体的保守同线性表明人类FUBP基因可能位于4 q上，如侧翼位点NFKB 1（4 q24）和ADH 1和ADH 3（4 q21-q23）所示。然而，Ddit 1的人类同源物，DNA损伤诱导转录本-1的DDIT 1，编码一种蛋白质，也称为GADD 45，已被分配到1 p31。2-p31 1（GDB）。因此，最有可能在该位置发现FUBP。
Results: The mouse Fubp gene was assigned to chromosome 3 by using a somatic cell hybrid panel. Its map location was further defined by typing the Jackson BSS 2 backcross panel, and Fubp was found to cosegregate with Ddit1 in all 94 animals analyzed (Fig. 1B). Thus, the Fubp gene was placed on the linkage map of mouse chromosome 3 at 70.5 cM from the centromere, in the distal end of mouse chromosome 3 (Fig. 1A).Additional comments: Searching the Mouse Genome Informatics Database (http://www. informatics. jax. org/locus. html) for mouse mutants near the Fubp locus revealed Va, varitint-waddler. Va is a semidominant mutation. Heterozygous Va mice are deaf and show circling behavior, headtossing, and hyperactivity. Their coats are variegated with patches of normal-colored, diluted, and white fur. Viability of heterozygotes is nearly normal, while mortality is very high in homozygotes, and very few of the survivors are fertile (1, 2). Fubp’s map position and its function as a nucleic acid binding protein and genetic regulator make it a potential candidate gene for this mutant. Homologies: Conserved synteny with human chromosomes would suggest a possible location of the human FUBP gene on 4q, as suggested by flanking loci NFKB1 (at 4q24) and ADH1 and ADH3 (at 4q21–q23). The human homologue of Ddit1, DDIT1 for DNA-damage induced transcript-1 encoding a protein also known as GADD45, however, has been assigned to 1p31. 2–p31. 1 (GDB). Therefore, it is most likely that FUBP will be found in that location.