Expression of human telomerase reverse transcriptase (hTERT) protein is significantly associated with the progression, recurrence and prognosis of oral squamous cell carcinoma in Taiwan
Expression of human telomerase reverse transcriptase (hTERT) protein is significantly associated with the progression, recurrence and prognosis of oral squamous cell carcinoma in Taiwan
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DOI：
10.1016/j.oraloncology.2006.01.011
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发表时间：
2007-02-01
期刊：
影响因子：
4.8
通讯作者：
Chiang, Chun-Pin
中科院分区：
文献类型：
--
作者：
Chen, Huang-Hsu;Yu, Chuan-Hang;Chiang, Chun-Pin
应用免疫组织化学方法检测82例口腔鳞癌、116例口腔上皮异常增生（OED）和21例正常口腔粘膜（NOM）组织中hTERT蛋白的表达。计算OSCC、OED和NOM样品中的细胞质和细胞核hTERT染色强度（SI; 0，无染色; 1，弱; 2，中等; 3，强）、标记指数（Ls，定义为阳性细胞在总细胞中的百分比）和标记评分（LS，定义为L1 × SI），并在组间进行比较。统计分析OSCC细胞质或细胞核hTERT LS与临床病理参数及患者生存期的相关性。从NOM（87 +/- 17%）到OED（95 +/- 18%）再到OSCC样本（114 +/-33%，p = 0.000），平均细胞质hTERT LS显着增加。平均细胞核hTERT LS也从NOM（80 +/- 14%）增加到OED（91 +/- 20%），然后降低到OSCC样本（86 +/- 35%），3组之间无统计学显著差异。在男性患者中，较高的平均细胞质hTERTLS与OSCC发生之间存在显著相关性（p = 0.023），肿瘤尺寸较大（T3和T4，p = 0.048），临床分期更晚期（3期和4期，p = 0.033），或来自嚼槟榔（p = 0.029）、吸烟（p = 0.027）或饮酒习惯（p = 0.025）的患者。此外，核hTERT LS大于100%的OSCC患者倾向于具有较高的复发率（p = 0.044）和较低的5年生存率（p = 0.011）。我们的研究结果表明，hTERT蛋白的表达增加是口腔癌发生的早期事件，hTERT可能是OSCC的生物标志物。在台湾，检测口腔鳞状细胞癌样本中hTERT的细胞质或细胞核表达量可以预测口腔癌的进展、复发和预后。(c)2006爱思唯尔有限公司保留所有权利。
This study used an immunohistochemical technique to examine the expression of human tetomerase reverse transcriptase (hTERT) protein in 82 specimens of OSCC, 116 specimens of oral epithelial dysplasia (OED), and 21 specimens of normal oral mucosa (NOM). The cytoplasmic and nuclear hTERT staining intensity (Sl; 0, no staining; 1, weak; 2, moderate; 3, strong), labeling indices (Lls, defined as the percentage of positive cells in total cells), and labeling scores (LSs, defined as Ll x Sl) in OSCC, OED, and NOM samples were calculated and compared among groups. The correlation between the cytoplasmic or nuclear hTERT LS in OSCCs and clinicopathological parameters or survival of OSCC patients was analyzed statistically. The mean cytoplasmic hTERT LSs increased significantly from NOM (87 +/- 17%) through OED (95 +/- 18%) to OSCC samples (114 +/- 33%, p = 0.000). The mean nuclear hTERT LSs also increased from NOM (80 +/- 14%) to OED (91 +/- 20%) and then decreased to OSCC samples (86 +/- 35%) with no statistically significant difference among the 3 groups. A significant correlation was found between the higher mean cytoplasmic hTERT LSs and OSCCs occurring in male-patients (p = 0.023), with larger tumor sizes (T3 and T4, p = 0.048), with more advanced clinical stages (stages 3 and 4, p = 0.033), or from patients with areca quid chewing (p = 0.029), cigarette smoking (p = 0.027), or alcohol drinking habit (p = 0.025). In addition, OSCC patients with nuclear hTERT LSs greater than 100% were prone to have a higher recurrence rate (p = 0.044) and a lower 5-year survival rate (p = 0.011). Our results indicate that the increased expression of hTERT protein is an early event in oral carcinogenesis and hTERT may be a biomarker for OSCCs. Measuring the amount of cytoplasmic or nuclear expression of hTERT in OSCC samples may predict the oral cancer progression, recurrence, and prognosis in Taiwan. (c) 2006 Elsevier Ltd. All rights reserved.