A 'hot-spot' for Ty transposition on the left arm of yeast chromosome III.
A 'hot-spot' for Ty transposition on the left arm of yeast chromosome III.
复制标题
酵母 III 号染色体左臂上 Ty 转座的“热点”。
DOI：
10.1093/nar/14.8.3475
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发表时间：
1986
影响因子：
14.9
通讯作者：
Oliver,SG
中科院分区：
文献类型：
--
作者：
Warmington,JR;Anwar,R;Newlon,CS;Waring,RB;Davies,RW;Indge,KJ;Oliver,SG
酵母染色体III的小环状衍生物由左臂的HML和右臂的HMR交叉形成，含有三个Ty元件。II类元件Ty 1-17位于LEU 2的左臂着丝粒远端，而两个I类元件串联排列在右臂PGK远端。这个区域的两侧是两个tRNA基因的5'端，tRNA 3Glu在着丝粒远端，tRNA 3Leu在LEU 2的正前方。在tRNA 3Glu基因附近，有一个区域含有以相反方向组织的简并δ序列。紧接着Ty 1-17的远端，有两个完整的单独的Delta元件，一个插入另一个。序列表明这两个δ序列通过单独的转座事件插入到染色体III中。提出了一个模型来解释这种结构是如何产生的，并讨论了转座子的传播和维护中的独奏三角洲元素的作用。
The small ring derivative ofSaccharomyces cerevisiaechromosome III, which was formed by a cross-over betweenHMLon the left arm andHMRon the right arm, contains three Ty elements. The class II element Ty 1-17 lies immediately centromere-distal toLEU2on the left arm while two class I elements are tandemly arranged distal toPGKon the right arm. We have sequenced the regions of chromosome III surrounding Ty 1-17 and have defined a region where a number of transposition events have occurred. This region is flanked by the 5' ends of two tRNA genes, tRNA3Glu on the centromere distal side and tRNA3Leu immediately in front ofLEU2. Close to the tRNA3Glu gene there is a region containing degenerate delta sequences organised in opposite orientations. Immediately distal to Ty 1-17 there are two complete solo delta elements, one inserted into the other. The sequence indicates that these two delta sequences were inserted into chromosome III by separate transposition events. A model is presented to explain how this structure arose and the role of solo delta elements in transposon propagation and maintenance is discussed.