Measurement of Uninterrupted Cerebral Blood Flow by Laser Speckle Contrast Imaging (LSCI) During the Mouse Middle Cerebral Artery Occlusion Model by an Inverted LSCI Setup.
Measurement of Uninterrupted Cerebral Blood Flow by Laser Speckle Contrast Imaging (LSCI) During the Mouse Middle Cerebral Artery Occlusion Model by an Inverted LSCI Setup.
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DOI：
10.1007/978-1-0716-2926-0_9
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发表时间：
2023-01-01
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通讯作者：
Marrelli, Sean P
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作者：
Hong, Sung-Ha;Doan, Andrea;Marrelli, Sean P
激光散斑对比成像(LSCI)提供了测量小鼠完整头骨的相对脑血流量(CBF)的能力。LSCI可用于检测大脑中动脉闭塞/再灌注(MCAO/R)卒中模型大脑皮质脑血流量的变化。然而，由于传统的LSCI方法被设计为从上方成像，因此在MCAO/R过程中不间断地测量CBF是不可能的，因为需要在俯卧位和仰卧位之间反复地重新定位鼠标。我们提出了一种改进的方法，从手术准备下进行LSCI测量，从而允许通过重新引入血流量从基线开始不间断地测量相对CBF。我们提供了一个3D可打印的成像平台和相应的头部框架，以及提高年轻和老年小鼠颅骨清晰度的方法。
Laser speckle contrast imaging (LSCI) offers the ability to measure relative cerebral blood flow (CBF) through the intact skull in mice. LSCI can be used to measure changes in cortical CBF in the middle cerebral artery occlusion/reperfusion (MCAo/R) stroke model. However, because conventional LSCI approaches are designed to image from above, uninterrupted measurement of CBF during the MCAo/R procedure is not possible due to the need to repeatedly reposition the mouse between prone and supine positions. We present a modified method to perform LSCI measurement from beneath the surgical preparation, thus allowing uninterrupted measurement of relative CBF from baseline through re-introduction of blood flow. We provide a 3D printable imaging platform and corresponding head frame, as well as methods to improve skull clarity in young and aged mice.