ISOLATION OF A NEUROPEPTIDE CORRESPONDING TO THE N-TERMINAL 27 RESIDUES OF THE PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE WITH 38 RESIDUES (PACAP38)
ISOLATION OF A NEUROPEPTIDE CORRESPONDING TO THE N-TERMINAL 27 RESIDUES OF THE PITUITARY ADENYLATE-CYCLASE ACTIVATING POLYPEPTIDE WITH 38 RESIDUES (PACAP38)
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DOI：
10.1016/0006-291x(90)92140-u
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发表时间：
1990-07-31
影响因子：
3.1
通讯作者：
ARIMURA, A
中科院分区：
文献类型：
--
作者：
MIYATA, A;JIANG, L;ARIMURA, A
使用大鼠垂体细胞培养物中的垂体腺苷酸环化酶活化作为生物活性的参数，从绵羊下丘脑组织中分离具有38个残基的新型神经肽（PACAP 38）（Miyata等人，Biochem.Biophys.Biophys.2004，2005）。通信资源164，567-574，1989）。从PACAP 38纯化过程中获得的侧馏分中，分离出具有27个残基的较短形式的肽，其对应于PACAP 38的N-末端27个氨基酸和酰胺化的C-末端，并命名为PACAP 27。合成的PACAP 27显示出与PACAP 38相当的腺苷酸环化酶刺激的生物活性。PACAP 27与血管活性肠肽（VIP）具有相当的同源性，其血管抑制活性与VIP相似，但腺苷酸环化酶活性比VIP高约1000倍。
A novel neuropeptide with 38 residues (PACAP38) was isolated from ovine hypothalamic tissues using the pituitary adenylate cyclase activation in rat pituitary cell cultures as a parameter of the biological activity (Miyata et al, Biochem. Biophys. Res. Commun. 164, 567-574, 1989). From the side fractions obtained during the purification of PACAP38, a shorter form peptide with 27 residues corresponding to the N-terminal 27 amino acids of PACAP38 and amidated C-terminus was isolated and named as PACAP27. Synthetic PACAP27 showed a biological activity of adenylate cyclase stimulation comparable to PACAP38. Moreover PACAP27 which shows a considerable homology with vasoactive intestinal polypeptide (VIP) demonstrated a similar vasodepressor activity as VIP, but the adenylate cyclase stimulating activity was about 1000 times greater than VIP.