Role of pulmonary alveolar macrophages in defense of the lung against Pseudomonas aeruginosa
Role of pulmonary alveolar macrophages in defense of the lung against Pseudomonas aeruginosa
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DOI：
10.1128/iai.68.8.4585-4592.2000
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发表时间：
2000-08-01
影响因子：
3.1
通讯作者：
Speert, DP
中科院分区：
文献类型：
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作者：
Cheung, DOY;Halsey, K;Speert, DP
肺泡巨噬细胞 (AM) 是下呼吸道抵御微生物入侵的第一道防线之一，评估了 AM 在小鼠肺内攻击后清除铜绿假单胞菌中的作用。通过鼻内给予脂质体封装的二氯亚甲基二膦酸酯来耗尽AM。在滴注脂质体后24小时，鼻内接种亚致死剂量的铜绿假单胞菌。然后评估脾、肝和肺组织中的活细菌和组织病理学，与对照组相比，AM 消耗 78% 至 88% 并不影响感染小鼠的存活率或铜绿假单胞菌从脾、肝或肺中的清除，但小鼠对肺炎克雷伯菌的易感性大大增强。中性粒细胞向肺部的募集也没有受到影响。新鲜移植的 AM 不能吞噬未经调理的铜绿假单胞菌，但能够吞噬酵母聚糖颗粒。进一步研究评估AM的原位吞噬活性，鼻内滴注铜绿假单胞菌或其他颗粒后3小时，进行支气管肺泡灌洗。 AM 对酵母聚糖颗粒和乳胶珠的吞噬作用超过了铜绿假单胞菌，中性粒细胞被招募到肺部以应对高剂量的细菌攻击。这些结果表明，AM 在保护肺部免受铜绿假单胞菌侵害方面并没有发挥重要作用。
Alveolar macrophages (AM) provide one of the first lines of defense against microbial invasion in the lower airways, The role of AM in the clearance of Pseudomonas aerrcginosa in mice after intrapulmonary challenge was evaluated. AM were depleted by intranasal administration of liposome-encapsulated dichloromethylene diphosphonate, At 24 h following the instillation of liposomes, a sublethal dose of P. aeruginosa was inoculated intranasally. Spleen, liver, and lung tissue was then evaluated for viable bacteria and for histopathology, AM depletion of 78 to 88% did not affect the survival rate of infected mice or clearance of P. aeruginosa from the spleen, liver, or lung, compared to the control group, but the mice's susceptibility to Klebsiella pneumoniae was greatly enhanced. The recruitment of neutrophils to the lung was also not affected. Freshly explanted AM were not competent to phagocytose unopsonized P. aeruginosa but were able to phagocytose zymosan particles. Further studies were conducted to assess the in situ phagocytic activities of AM, Three hours after the intranasal instillation of P. aeruginosa or other particles, bronchoalveolar lavage was performed. AM phagocytosis of zymosan particles and latex beads exceeded that of P. aeruginosa, Neutrophils were recruited to the lung in response to a high-dose bacterial challenge. These results suggest that AM do not play an important role in defense of the lung against P. aeruginosa.