Quantitative trait loci affecting life span in replicated popidations of Drosophila melanogaster.: I.: Composite interval mapping
Quantitative trait loci affecting life span in replicated popidations of Drosophila melanogaster.: I.: Composite interval mapping
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DOI：
10.1534/genetics.103.023218
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发表时间：
2004-09-01
期刊：
影响因子：
3.3
通讯作者：
Service, PM
中科院分区：
文献类型：
--
作者：
Forbes, SN;Valenzuela, RK;Service, PM
利用复合区间作图法对3个不同自交系间杂交的F2代群体进行寿命QTL定位。每个近交系都来自不同的远交群体，这些远交群体经历了长寿命或短寿命的长期选择。以微卫星位点为遗传标记，采用Bootstrapping法估计QTL定位的置信区间。检测到至少10个QTL，其中9个位于两个主要的amosonres。5个QTL存在于至少两个杂交组合中，5个QTL存在于两性中。在多个杂交中观察到相同的QTL与寿命的遗传变异通过平衡选择维持的假设一致。除一个QTL外，所有QTL的等位基因效应均与远交源群体预测的方向一致。赋予较长寿命的等位基因总是至少部分占优势。
Composite interval mapping was used to identify life-span QTL in F-2 progeny of three crosses between different pairs of inbred lines. Each inbred line was derived from a different outbred population that had undergone long-term selection for either long or short life span. Microsatellite loci were used as genetic markers, and confidence intervals for QTL location were estimated by bootstrapping. A minimum of 10 QTL were detected, nine of which were located on the two major amosonres. Five QTL were present in at least two crosses and five were present in both sexes. Observation of the same QTL in more than one cross was consistent with the hypothesis that genetic variation for life span is maintained by balancing selection. For all QTL except one, allelic effects were in the direction predicted on the basis of outbred source population. Alleles that conferred longer life were always at least partially dominant.