Low, but Not High, Doses of Cisplatin Damage Cochlear Hair Cells in C57 Mouse Organotypic Cultures
Low, but Not High, Doses of Cisplatin Damage Cochlear Hair Cells in C57 Mouse Organotypic Cultures
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低剂量但不高剂量的顺铂会损害 C57 小鼠器官培养物中的耳蜗毛细胞
DOI：
10.1159/000446189
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发表时间：
2016-06
影响因子：
1.3
通讯作者：
Yin Shankai
中科院分区：
文献类型：
--
作者：
Zeng Shan;Sun Xiaoqiang;Chen Zhengnong;Yu Dongzhen;Chen Bin;Yin Shankai
目的：探讨体外顺铂诱导C57小鼠耳蜗毛细胞损伤的特点。方法：取2~4日龄C57小鼠耳蜗47个。40例标本用不同浓度的顺铂(10、25、50、100、400、1000μ/L)处理48h，其余7例作为对照组。结果：顺铂浓度为10~100μμ/L时，毛细胞脱落率由14.5%增加到78.4%，400和1000 Mol/L时毛细胞脱落率分别降至48.8%和8.77%。DAPI染色检测毛细胞损伤区的细胞凋亡。毛细胞损失率在顺铂治疗组之间有显著差异。顺铂剂量与毛细胞数的线性回归分析显示，顺铂剂量达100μμ/L时，毛细胞数与顺铂剂量呈显著负相关，而当顺铂剂量达1,000μ/L时，毛细胞数与顺铂剂量呈正相关。结论：顺铂诱导的毛细胞损伤仅在一定剂量范围内呈浓度依赖性，高剂量顺铂可引起耳蜗细胞的损伤抵抗。
Aims: The purpose of this study was to investigate the characteristics of cisplatin-induced C57 mouse cochlear hair cell damage in vitro. Methods: Forty-seven cochleae harvested from 2- to 4-day-old C57 mice were used. Forty specimens were treated with different concentrations of cisplatin (10, 25, 50, 100, 400, and 1,000 μmol/l) for 48 h. The remaining seven specimens were used as a control group. Results: The rate of hair cell loss increased from 14.5 to 78.4% over cisplatin concentrations of 10 to 100 μmol/l, whereas hair cell loss decreased to 48.8 and 8.77% at concentrations of 400 and 1,000 μmol/l, respectively. Apoptosis was detected by DAPI staining in the areas of hair cell damage. Hair cell loss rates differed significantly among the cisplatin-treated groups. Linear regression analysis of cisplatin dose versus hair cell number showed a significant negative correlation for cisplatin doses up to 100 μmol/l and a positive correlation with further increases up to 1,000 μmol/l. Conclusions: We conclude that cisplatin-induced hair cell damage was concentration dependent only up to a certain dose and that injury resistance may occur in cochlear cells treated with higher doses of cisplatin.
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影响因子：
2.8
作者：
Kawamoto K;Izumikawa M;Beyer LA;Atkin GM;Raphael Y
通讯作者：
Raphael Y
影响因子：
3.3
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van de Sluis, B;Rothuizen, J;Wijmenga, C
通讯作者：
Wijmenga, C
影响因子：
6
作者：
Ciarimboli, Giuliano;Deuster, Dirk;Schlatter, Eberhard
通讯作者：
Schlatter, Eberhard