Chromosomal localization of angiotensin II type 1 receptor isoforms in the rat.
Chromosomal localization of angiotensin II type 1 receptor isoforms in the rat.
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DOI：
10.1006/bbrc.1993.1875
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发表时间：
1993-07
影响因子：
3.1
通讯作者：
J. Lewis;T. Serikawa;D. Warnock
中科院分区：
文献类型：
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作者：
J. Lewis;T. Serikawa;D. Warnock
大鼠血管紧张素II 1型受体的两种不同亚型AT1A和AT1B的cDNA序列已被报道。使用单组聚合酶链反应引物从大鼠基因组DNA中扩增AT1A和AT1B的序列。然后用这些引物扩增来自一组大鼠x小鼠体细胞杂交细胞系的基因组DNA，所述细胞系已被表征为大鼠染色体含量。从大鼠AT1A和AT1B的扩增产物彼此区分，并通过使用不同的限制性图谱的小鼠。使用该方法，AT1A定位于大鼠17号染色体，AT1B定位于大鼠2号染色体。
The cDNA sequences of two different isoforms of the rat angiotensin II type 1 receptors, AT1A and AT1B, have been reported. A single set of polymerase chain reaction primers was used to amplify sequence from both AT1A and AT1B from rat genomic DNA. Genomic DNA from a panel of rat x mouse somatic hybrid cell lines which had been characterized as to the rat chromosomal content was then amplified with these primers. The amplified products from rat AT1A and AT1B were distinguished from each other and those of the mouse by the use of differential restriction patterns. Using this method, AT1A was localized to rat chromosome 17 and AT1B to rat chromosome 2.