Hemopoietic and angiogenetic progenitors in healthy athletes: different responses to endurance and maximal exercise
Hemopoietic and angiogenetic progenitors in healthy athletes: different responses to endurance and maximal exercise
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DOI：
10.1152/japplphysiol.01344.2009
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发表时间：
2010-07-01
影响因子：
3.3
通讯作者：
Testa, Ugo
中科院分区：
文献类型：
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作者：
Bonsignore, Maria R.;Morici, Giuseppe;Testa, Ugo
Bonsignore MR，Morici G，Riccioni R，Huertas A，Petrucci E，Veca M，马里亚尼G，Bonanno A，Chimenti L，Gioia M，Palange P，Testa U.健康运动员的造血和血管生成祖细胞：对耐力和极限运动的不同反应。J Appl Physiol 109：60-67，2010.首次发表于2010年5月6日; doi：10.1152/japplphysiol.01344.2009.-耐力或极限运动对健康受试者骨髓造血和血管生成祖细胞动员的影响尚不清楚。在10名健康的业余跑步者中，我们收集了马拉松比赛前、结束时和比赛后第二天（n = 9）以及1.5公里野外测试前和结束时（n = 8）的静脉血，并通过流式细胞术和培养分析测量造血和血管生成祖细胞，以及血浆或血清中几种细胞因子/生长因子的浓度。在马拉松后，CD 34（+）细胞没有变化，而克隆生成分析显示红细胞（BFU-E）和粒细胞-单核细胞（CFU-GM）系列的集落数量减少，在比赛后早晨恢复到基线水平。相反，CD 34（+）细胞、BFU-E和CFU-GM在田间试验后增加。血管生成祖细胞，评估为CD 34（+）KDR（+）和CD 133（+）VE-钙粘蛋白（+）细胞或表达内皮标记物的培养物中的贴壁细胞，在耐力和最大运动后增加，但在方案之间显示不同的模式。白细胞介素-6增加更多的马拉松后比现场测试后，而肝细胞生长因子和干细胞因子增加相似，在两个协议。两种类型的运动后，血浆血管生成素（Ang）1和2的水平均升高，而Ang-1与Ang-2的比率或血管内皮生长因子-A几乎没有受到影响。这些数据表明，循环造血祖细胞可能被利用在外周组织在长时间的耐力运动。健康受试者运动后内皮祖细胞的动员似乎受到运动类型的调节。运动诱导的生长因子增加表明运动对骨髓有生理营养作用。
Bonsignore MR, Morici G, Riccioni R, Huertas A, Petrucci E, Veca M, Mariani G, Bonanno A, Chimenti L, Gioia M, Palange P, Testa U. Hemopoietic and angiogenetic progenitors in healthy athletes: different responses to endurance and maximal exercise. J Appl Physiol 109: 60-67, 2010. First published May 6, 2010; doi:10.1152/japplphysiol.01344.2009.-The effects of endurance or maximal exercise on mobilization of bone marrow-derived hemopoietic and angiogenetic progenitors in healthy subjects are poorly defined. In 10 healthy amateur runners, we collected venous blood before, at the end of, and the day after a marathon race (n = 9), and before and at the end of a 1.5-km field test (n = 8), and measured hemopoietic and angiogenetic progenitors by flow cytometry and culture assays, as well as plasma or serum concentrations of several cytokines/growth factors. After the marathon, CD34(+) cells were unchanged, whereas clonogenetic assays showed decreased number of colonies for both erythropoietic (BFU-E) and granulocyte-monocyte (CFU-GM) series, returning to baseline the morning post-race. Conversely, CD34(+) cells, BFU-E, and CFU-GM increased after the field test. Angiogenetic progenitors, assessed as CD34(+)KDR(+) and CD133(+)VE-cadherin(+) cells or as adherent cells in culture expressing endothelial markers, increased after both endurance and maximal exercise but showed a different pattern between protocols. Interleukin-6 increased more after the marathon than after the field test, whereas hepatocyte growth factor and stem cell factor increased similarly in both protocols. Plasma levels of angiopoietin (Ang) 1 and 2 increased after both types of exercise, whereas the Ang-1-to-Ang-2 ratio or vascular endothelial growth factor-A were little affected. These data suggest that circulating hemopoietic progenitors may be utilized in peripheral tissues during prolonged endurance exercise. Endothelial progenitor mobilization after exercise in healthy trained subjects appears modulated by the type of exercise. Exercise-induced increase in growth factors suggests a physiological trophic effect of exercise on the bone marrow.