A novel comprehensive analysis method for Staphylococcus aureus pathogenicity islands
A novel comprehensive analysis method for Staphylococcus aureus pathogenicity islands
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DOI：
10.1111/1348-0421.12007
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发表时间：
2013-02-01
影响因子：
2.6
通讯作者：
Hu, Dong-Liang
中科院分区：
文献类型：
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作者：
Sato'o, Yusuke;Omoe, Katsuhiko;Hu, Dong-Liang
金黄色葡萄球菌致病岛（Staphylococcus aureus pathogenicity islands，SaPI）是葡萄球菌中一个不断发展的移动的遗传元件家族。MGEs的水平遗传转移在S.金黄色。几种SaPI携带葡萄球菌肠毒素和SE样毒素基因。为了全面研究SaPI基因的多样性，设计了一系列引物，对应于S.设计了金黄色葡萄球菌基因组，建立了一种长时间、准确的（LA）-PCR分析方法。在携带seb、selk和selq基因的菌株中，LA-PCR扩增产物为1317 kbp。限制性片段长度多态性（RFLP）分析表明，该产品具有不同的RFLP特征比以前描述的SaPI，因此，他们预计将包括新的SaPI。核苷酸测序分析显示了7种新型SaPI：seb-携带SaPIivm 10、SaPishikawa 11、SaPIivm 60、SaPIno 10和SaPIhirosaki 4、selk和selq-携带SaPIj 11和非超抗原-携带SaPIhhms 2。这些SaPI具有镶嵌结构，包含已知SaPI和其他未知基因的组分。发现携带不同SaPI的菌株具有显著不同的超抗原毒素产生。本研究结果表明，LA-PCR方法可以全面地鉴定SaPI多样性，并有助于研究S.金黄色葡萄球菌致病性
Staphylococcus aureus pathogenicity islands (SaPIs) form a growing family of mobile genetic elements (MGEs) in Staphylococci. Horizontal genetic transfer by MGEs plays an important role in the evolution of S. aureus. Several SaPIs carry staphylococcal enterotoxin and SE-like toxin genes. To comprehensively investigate the diversity of SaPIs, a series of primers corresponding to sequences flanking six SaPI insertion sites in S. aureus genome were designed and a long and accurate (LA)-PCR analysis method established. LA-PCR products of 1317kbp were observed in strains with seb, selk or selq genes. Restriction fragment length polymorphism (RFLP) analysis showed that the products have different RFLP characteristics than do previously described SaPIs; they were therefore predicted to include new SaPIs. Nucleotide sequencing analysis revealed seven novel SaPIs: seb-harboring SaPIivm10, SaPishikawa11, SaPIivm60, SaPIno10 and SaPIhirosaki4, selk and selq-harboring SaPIj11 and non-superantigen-harboring SaPIhhms2. These SaPIs have mosaic structures containing components of known SaPIs and other unknown genes. Strains carrying different SaPIs were found to have significantly different production of superantigen toxins. The present results show that the LA-PCR approach can comprehensively identify SaPI diversity and is useful for investigating the evolution of S. aureus pathogenicity.