Considerations for Accurate Identification of Adult Culex restuans (Diptera: Culicidae) in Field Studies
Considerations for Accurate Identification of Adult Culex restuans (Diptera: Culicidae) in Field Studies
复制标题
野外研究中准确识别雷氏库蚊成虫（双翅目：库蚊科）的注意事项
DOI：
--
复制
发表时间：
2008
影响因子：
2.1
通讯作者：
R. Poulson
中科院分区：
文献类型：
--
作者：
L. Harrington;R. Poulson
摘要 了解不同蚊种（双翅目：蚊科）的生态和行为对于确定它们在疾病传播周期和公共卫生风险中的作用至关重要。美国东北部的两种库蚊（Culex pipiens L. 和 Culex Restuans Theobald）与西尼罗河病毒（黄病毒科、黄病毒属，WNV）的地方性传播有关。尽管区分这两个物种的成虫很困难，但许多公共卫生工作者和媒介生物学家在现场收集成虫，根据外部形态将这些物种分开。由于时间或成本限制，通常使用这种方法，而不是检查解剖的男性生殖器或基于聚合酶链反应 (PCR) 的诊断。我们通过将盲法评分形态与基于 PCR 的确认进行比较，评估了七个已发表的形态特征的可靠性，以区分这些物种的成虫。我们的研究表明 Cx 的形态学鉴定。 pipiens 对于 Cx 来说是边缘性的并且通常不可靠。恢复。我们还使用基于分子的方法检查了错误率。 DNA 样本中甚至有来自另一个物种的一条腿的污染。我们的结论是，为了充分了解库蚊物种在西尼罗河病毒和其他病原体流行病学中各自的作用，应该更多地关注这些因素，以进行准确的物种鉴定。
Abstract Understanding the ecology and behavior of different mosquito species (Diptera: Culicidae) is essential for identifying their role in disease transmission cycles and public health risk. Two species of Culex mosquitoes in the northeastern United States, Culex pipiens L. and Culex restuans Theobald, have been implicated in enzootic transmission of West Nile virus (family Flaviviridae, genus Flavivirus, WNV). Despite the difficulty of differentiating these two species as adults, many public health workers and vector biologists collecting adults in the field separate these species based on external morphology. This approach is often used rather than examination of dissected male genitalia or polymerase chain reaction (PCR)-based diagnostics due to time or cost constraints. We evaluated the reliability of seven published morphological characters to differentiate adults of these species by comparing blindly scored morphology with PCR-based confirmations. Our study demonstrates that morphological identification of Cx. pipiens is marginal and often not reliable for Cx. restuans. We also examined error rates with molecular-based approaches. DNA samples were contaminated with as little as one leg from another species. We conclude that to fully understand the respective roles of Culex species in the epidemiology of WNV and other pathogens, more attention should be paid to these considerations for accurate species identification.
影响因子：
2.7
作者：
Brody, JR;Kern, SE
通讯作者：
Kern, SE