High uptake carboxyl-functionalized porous beta-cyclodextrin polymer for selective extraction of lysozyme from egg white
High uptake carboxyl-functionalized porous beta-cyclodextrin polymer for selective extraction of lysozyme from egg white
复制标题
高吸收羧基功能化多孔β-环糊精聚合物用于从蛋清中选择性提取溶菌酶
DOI：
10.1016/j.chroma.2019.04.056
复制
发表时间：
2019
影响因子：
4.1
通讯作者：
Zhang Zhi Qi
中科院分区：
文献类型：
--
作者：
Duan Hui Ling;Niu Qi Le;Wang Jun;Ma Shi Yao;Zhang Jing;Zhang Zhi Qi
溶菌酶因其具有的溶菌作用而被广泛应用于医疗、食品和工业领域，因此设计和开发一种对溶菌酶具有高吸附容量和高选择性的专用吸附剂具有重要意义。受四氟对苯二甲腈交联的多孔β-环糊精聚合物(P-CDPS)的高吸附能力和溶菌酶与羧基之间的非共价相互作用的启发，通过碱催化将P-CDPS中的氰基水解为羧基，合成了羧基功能化的P-CDPS(P-CDP-COO−)。P-CDP-CoO−的孔道结构、大范围的羧基、环糊精的阻聚性以及带负电荷等特点使其对溶菌酶具有优异的吸附能力。最大饱和吸附容量达到1520 mg g−1，大大优于母体聚合物和其他文献报道的材料。将所制备的材料成功地用于蛋清中溶菌酶的选择性提取。
Lysozyme is widely used in medical, food and industrial fields due to its bacteriolytic effect and thus it is significant to design and develop a specific adsorbent with high adsorption capacity and selectivity for lysozyme. Inspired by the high uptake capacity of tetrafluoroterephthalonitrile-crosslinked porous β-cyclodextrin polymers (P-CDPs) and the noncovalent interaction between lysozyme and carboxyl groups, the carboxyl-functionalized P-CDPs (P-CDP-COO−) were synthesized by base-catalysed hydrolysis of nitrile group in P-CDPs to carboxyl. Porous structure, large extent of carboxylic functional groups, cyclodextrin's preventing aggregation, and negatively charged make P-CDP-COO−possess an outstanding adsorption capability for lysozyme. The maximum saturated adsorption capacity reaches 1520 mg g−1, which is much better than the parent polymer and other reported materials. The as-prepared material was successfully utilized for the selective extraction of lysozyme from egg white.