Producibility of exfoliative toxin and staphylococcal coagulase types of Staphylococcus aureus strains isolated from skin infections and atopic dermatitis.
Producibility of exfoliative toxin and staphylococcal coagulase types of Staphylococcus aureus strains isolated from skin infections and atopic dermatitis.
复制标题
从皮肤感染和特应性皮炎中分离出的金黄色葡萄球菌菌株的剥脱性毒素和葡萄球菌凝固酶类型的生产能力。
DOI：
10.1159/000245676
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发表时间：
1997
期刊：
影响因子：
3.4
通讯作者：
S. Kanzaki
中科院分区：
文献类型：
--
作者：
H. Kanzaki;M. Ueda;Y. Morishita;H. Akiyama;J. Arata;S. Kanzaki
背景
引起葡萄球菌烫伤样皮肤综合征（SSSS）和大疱性脓疱病的金黄色葡萄球菌菌株分泌剥脱毒素（ET）。已经报道了两种抗原性不同的ET血清型，ETA和ETB。
材料和方法
283株S.对从脓疱病、SSSS、疖（包括疖）和特应性皮炎中分离的金黄色葡萄球菌进行了ET产生、ET血清型和凝固酶型的检测。我们检查ET生产和ET血清型使用聚合酶链反应与寡核苷酸引物的eta和etb。
结果
ET产生者在脓疱病中的发生率为69.4%（100/144），在SSSS中为100%（6/6），在特应性皮炎中为2.8%（3/112），在疖中为0%（0/21）。单独的ETA由57株来自脓疱病的菌株和3株来自特应性皮炎的菌株产生。来自脓疱病的36个菌株和来自SSSS的所有6个菌株单独产生ETB。7株肺炎链球菌同时产生ETA和ETB。大多数产ETA的菌株属于凝固酶V型，大多数产ETB的菌株属于凝固酶I型。脓疱病菌株多为I型或V型，SSSS菌株均为I型。在S.金黄色葡萄球菌分离物来自疖。
结论
这些结果补充了关于S.金黄色葡萄球菌菌株分离自脓疱病、SSSS、疖病和特应性皮炎。我们发现ET血清型与产ET菌株的凝固酶型之间存在相关性。
BACKGROUND
Strains of Staphylococcus aureus which cause staphylococcal scalded skin syndrome (SSSS) and bullous impetigo secrete exfoliative toxin (ET). Two antigenically distinct serotypes of ET, ETA and ETB, have been reported.
MATERIALS AND METHODS
Two hundred eighty-three strains of S. aureus isolated from impetigo, SSSS, furuncles (including furunculosis) and atopic dermatitis were examined in terms of the producibility of ET, ET serotypes and coagulase types. We examined ET production and ET serotypes using the polymerase chain reaction with the oligonucleotide primers for eta and etb.
RESULTS
The incidence of ET producers was 69.4% (100/144) in impetigo, 100% (6/6) in SSSS, 2.8% (3/112) in atopic dermatitis and 0% (0/21) in furuncles. ETA alone was produced by 57 strains from impetigo and by 3 strains from atopic dermatitis. ETB alone was produced by 36 strains from impetigo and by all 6 strains from SSSS. Seven strains from impetigo produced both ETA and ETB. Most ETA producers belonged to coagulase type V and most ETB producers to coagulase type I. Impetigo strains mostly belonged to type I or V. All strains from SSSS were classified as type I. Type IV was most frequent among S. aureus isolates from furuncles.
CONCLUSION
These results add to the epidemiological information as to ET producibility and ET serotypes of S. aureus strains isolated from impetigo, SSSS, furunculosis and atopic dermatitis. We have found that there is a relationship between the ET serotypes and the coagulase types of ET-producing strains.