Phorbol 12-myristate 13-acetate (PMA) suppresses high Ca2+-enhanced adipogenesis in bone marrow stromal cells
Phorbol 12-myristate 13-acetate (PMA) suppresses high Ca2+-enhanced adipogenesis in bone marrow stromal cells
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佛波醇 12-肉豆蔻酸酯 13-乙酸酯 (PMA) 抑制骨髓基质细胞中高 Ca2 增强的脂肪生成
DOI：
10.1007/s12576-019-00690-9
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发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Katoh Youichi
中科院分区：
文献类型：
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作者：
Hashimoto Ryota;Miyamoto Yuki;Itoh Seigo;Daida Hiroyuki;Okada Takao;Katoh Youichi
我们之前报道过细胞外和细胞内Ca2+的增加导致骨髓基质细胞（BMSCs）中脂肪细胞的积累。然而，抑制高Ca2+增强脂肪细胞积累的策略尚未报道。研究了二酰基甘油类似物12-肉豆蔻酸13-乙酸酯（PMA）对小鼠原代骨髓间充质干细胞增殖和脂肪形成的影响。我们分别使用9 mM cacl2和100 nM离子霉素来增加细胞外Ca2+和细胞内Ca2+。在正常脂肪形成、脂肪形成+ CaCl2和脂肪形成+离子霉素条件下，PMA抑制C/EBPα和PPARγ的表达。PMA在正常脂肪形成条件下促进细胞增殖，而在脂肪形成+ cac2和脂肪形成+离子霉素条件下抑制细胞增殖。在正常脂肪形成条件下，PMA不影响脂肪细胞的积累，但在脂肪形成+ cac2和脂肪形成+离子霉素条件下，PMA抑制脂肪细胞的积累。这些结果表明，pma依赖途径是抑制高Ca2+增强脂肪细胞积累的重要信号通路。
We have previously reported that increased extracellular and intracellular Ca2+lead to adipocyte accumulation in bone marrow stromal cells (BMSCs). However, strategies to suppress high Ca2+-enhanced adipocyte accumulation have not been reported. We examined the effects of the diacylglycerol analog phorbol 12-myristate 13-acetate (PMA) on proliferation and adipogenesis of mouse primary BMSCs. We used 9 mM CaCl2and 100 nM ionomycin to increase extracellular Ca2+and intracellular Ca2+, respectively. PMA suppressed the expression of both C/EBPα and PPARγ under normal adipogenesis, adipogenesis + CaCl2, and adipogenesis + ionomycin conditions. PMA enhanced proliferation under normal adipogenesis conditions but suppressed proliferation under adipogenesis + CaCl2and adipogenesis + ionomycin conditions. PMA did not affect the accumulation of adipocytes under normal adipogenesis conditions but suppressed adipocyte accumulation under adipogenesis + CaCl2and adipogenesis + ionomycin conditions. These results suggest that the PMA-dependent pathway is an important signaling pathway to suppress high Ca2+-enhanced adipocyte accumulation.