Hierarchy of human IgG recognition within the Staphylococcus aureus immunome.
Hierarchy of human IgG recognition within the Staphylococcus aureus immunome.
复制标题
金黄色葡萄球菌免疫组中人类 IgG 识别的层次结构。
DOI：
10.1038/s41598-018-31424-3
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发表时间：
2018
影响因子：
4.6
通讯作者：
Silverman,GreggJ
中科院分区：
文献类型：
--
作者：
Radke,EmilyE;Brown,StuartM;Pelzek,AdamJ;Fulmer,Yi;Hernandez,DavidN;Torres,VictorJ;Thomsen,IsaacP;Chiang,WilliamK;Miller,AndyO;Shopsin,Bo;Silverman,GreggJ
金黄色葡萄球菌是一种机会性病原体，其菌株对抗生素的耐药性越来越强，可引起一系列与显著发病率相关的严重感染。然而，迄今为止，所有候选疫苗都未能在人体中诱导保护性免疫反应。我们需要对人类感染中重要的抗原靶点有更全面的了解。为了调查感染相关的免疫反应，从三种类型的临床感染中抽取患者在初次就诊和康复期间的样本；皮肤和软组织感染（SSTI），假体关节感染（PJI）和儿童血液性骨髓炎（PHO）。用一系列重组蛋白检测血清IgG的反应性，这些重组蛋白代表了来自社区获得性甲氧西林耐药的2652个体外翻译开放阅读框（orf）。aureusUSA300压力。在所有患者样本中，104种蛋白与血清IgG表现出高水平的反应性。总的来说，高水平的igg反应性通常是针对分泌蛋白的一个子集。尽管基于有限的调查，我们发现了s。金黄色葡萄球菌蛋白对不同临床综合征患者血清样品具有不同的反应性。总之，我们的研究揭示了这些不同蛋白质的层次结构。金黄色葡萄球菌“免疫组”，这将有助于推进努力开发保护性免疫治疗剂。
Staphylococcus aureusis an opportunistic pathogen that causes a range of serious infections associated with significant morbidity, by strains increasingly resistant to antibiotics. However, to date all candidate vaccines have failed to induce protective immune responses in humans. We need a more comprehensive understanding of the antigenic targets important in the context of human infection. To investigate infection-associated immune responses, patients were sampled at initial presentation and during convalescence from three types of clinical infection; skin and soft tissue infection (SSTI), prosthetic joint infection (PJI) and pediatric hematogenous osteomyelitis (PHO). Reactivity of serum IgG was tested with an array of recombinant proteins, representing over 2,652in-vitro-translated open reading frames (ORFs) from a community-acquired methicillin-resistantS.aureusUSA300 strain. High-level reactivity was demonstrated for 104 proteins with serum IgG in all patient samples. Overall, high-level IgG-reactivity was most commonly directed against a subset of secreted proteins. Although based on limited surveys, we found subsets ofS.aureusproteins with differential reactivity with serum samples from patients with different clinical syndromes. Together, our studies have revealed a hierarchy within the diverse proteins of theS.aureus“immunome”, which will help to advance efforts to develop protective immunotherapeutic agents.