Hyaluronic acid secreted by mesothelial cells: a natural barrier to ovarian cancer cell adhesion
Hyaluronic acid secreted by mesothelial cells: a natural barrier to ovarian cancer cell adhesion
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间皮细胞分泌的透明质酸：卵巢癌细胞粘附的天然屏障
DOI：
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发表时间：
1995
影响因子：
4
通讯作者：
G. Turner
中科院分区：
文献类型：
--
作者：
L. Jones;M. J. Gardner;J. Catterall;G. Turner
在多孔板中研究了8种培养的卵巢肿瘤细胞系与间皮细胞单层的粘附，作为腹膜腔中卵巢癌相互作用的一些模型。当仅抽吸来自汇合的间皮细胞培养物的条件培养基（CM）的上半部分时，肿瘤细胞的粘附性低（3.5%-36%）。当介质被完全去除时，粘附力增加。显示出最大粘附增强的肿瘤细胞系是先前已显示出表达最高量的CD 44的那些。通过向间皮细胞中加入红细胞悬液，发现间皮细胞周围有一层细胞周被膜，可以通过抽吸培养基或用透明质酸酶（Hase）处理培养基来破坏。用Hase处理CM也大大增加了肿瘤细胞粘附。此外，CM被证明含有大量的透明质酸（HA）。HA阻止在没有CM的情况下粘附，但效果不如细胞周涂层产生的效果大。建议间皮细胞产生的细胞周HA在卵巢肿瘤细胞在腹膜腔的侵袭中具有重要作用。
The adhesion to mesothelial monolayers of eight cultured ovarian tumour cell lines was studied in multiwell plates as a model for some of the interactions of ovarian cancer in the peritoneal cavity. When only the upper half of the conditioned medium (CM) from a confluent mesothelial cell culture was aspirated, the adhesion of the tumour cells was low (3.5%–36%). When the medium was removed completely the adhesion increased. The tumour cell lines showing the greatest enhancement of adhesion were those which had previously been shown to express the highest amounts of CD44. By adding erythrocyte suspensions to mesothelial cells it was shown that there was a pericellular coat around the mesothelial cells that could be destroyed by aspirating the medium, or by treating the medium with hyaluronidase (Hase). Treatment of the CM with Hase also considerably increased tumour cell adhesion. Furthermore, CM was shown to contain high amounts of hyaluronic acid (HA). HA blocked adhesion in the absence of CM, but the effect was not as large as that produced by the pericellular coat. It is proposed that pericellular HA produced by mesothelial cells has an important role in the invasion of ovarian tumour cells in the peritoneal cavity.
影响因子：
3.7
作者：
NIEDBALA, MJ;CRICKARD, K;BERNACKI, RJ
通讯作者：
BERNACKI, RJ
影响因子：
11.2
作者：
Stephen A. Cannistra;G. Kansas;J. Niloff;B. DeFranzo;Young Ho Kim;Christian H. Ottensmeier
通讯作者：
Stephen A. Cannistra;G. Kansas;J. Niloff;B. DeFranzo;Young Ho Kim;Christian H. Ottensmeier