The canine eye: in vitro dissolution of the barriers to aqueous outflow.
The canine eye: in vitro dissolution of the barriers to aqueous outflow.
复制标题
犬眼：体外溶解房水流出的障碍。
DOI：
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发表时间：
1978
影响因子：
4.4
通讯作者：
J. Brett
中科院分区：
文献类型：
--
作者：
E. V. Van Buskirk;J. Brett
在新鲜切除的犬眼中，通过恒压定量水灌注来评价其透明质酸酶敏感性和透明质酸酶抗性组分的房水流出设施和时间依赖性变化。平均基线流出便利度为0.24微升/分钟/毫米汞柱。随着在恒定眼内压下的长时间灌注，观察到流出设施几乎线性增加至少3小时，并继续增加长达10小时，达到最大值几倍于最初测量的设施。用合并的犬房水灌注不能防止测量设施的时间依赖性增加。快速交换前房内容物与灌注溶液单独产生立即增加三倍的设施，再次由一个逐渐的时间依赖性设施增加。前房内容物与透明质酸酶的快速交换产生了立即五倍的设施与稳定的测量设施超过3小时和随后的灌注。流出或“冲洗现象”的测量设施的时间依赖性变化似乎是由于在犬眼中的房水流出屏障的透明质酸酶敏感组分的逐渐溶解。
Facility of aqueous outflow and time-dependent changes in its hyaluronidase-sensitive and hyaluronidase-resistant components were evaluated in freshly excised canine eyes by constant pressure quantitative aqueous perfusion. Mean baseline facility of outflow was 0.24 microliter/min/mm Hg. With prolonged perfusion at constant intraocular pressure, facility of outflow was observed to increase almost linearly for at least 3 hr and continued to increase for up to 10 hr, reaching a maximum several times the initially measured facility. Perfusion with pooled dog aqueous humor did not prevent the time-dependent increase in measured facility. Rapid exchange of anterior chamber contents with perfusion solution alone produced an immediate threefold increase in facility, again followed by a gradual time-dependent facility increase. Rapid exchange of anterior chamber contents with hyaluronidase produced an immediate fivefold increase in facility with stabilization of measured facility over 3 hr and subsequent perfusion. The time-dependent changes in measured facility of outflow or "washout phenomenon" appeared to result from the gradual dissolution of the hyaluronidase-sensitive component of the barriers to aqueous outflow in the canine eye.