Exposure of pig oocytes to PCBs during in vitro maturation: effects on developmental competence, cytoplasmic remodelling and communications with cumulus cells.
Exposure of pig oocytes to PCBs during in vitro maturation: effects on developmental competence, cytoplasmic remodelling and communications with cumulus cells.
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DOI：
10.4081/907
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发表时间：
2004-10
期刊：
影响因子：
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通讯作者：
T. Brevini;R. Vassena;A. Paffoni;C. Francisci;U. Fascio;F. Gandolfi
中科院分区：
文献类型：
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作者：
T. Brevini;R. Vassena;A. Paffoni;C. Francisci;U. Fascio;F. Gandolfi
多氯联苯 (PCB) 是生态系统中最持久、最广泛的内分泌干扰化合物之一。这些物质存在于污水污泥中，这些污泥作为肥料越来越多地撒在耕地和牧场上，并被农场动物通过食物和饮用水摄入。本研究探讨了不同PCB浓度对猪卵母细胞体外成熟和发育能力的影响，并探讨了可能的机制。将浓度范围为 0 至 1 mg/mL 的 Aroclor 1254 (A1254)（包含 60 多种 PCB 同系物）添加到成熟培养基中，因为其成分被认为与环境相关。在任何测试剂量下，A1254 对猪卵母细胞的成熟以及孤雌激活后分裂的卵母细胞的数量没有影响。相比之下，在 10 ng/mL 的浓度下，发育至囊胚阶段的受精卵数量明显减少。获得的囊胚数量显着减少，并且随着浓度的增加呈剂量反应方式。暴露于多氯联苯会改变线粒体在成熟过程中的重新定位，这与细胞质微管网络的缺乏有关。没有观察到对线粒体活性的影响。 A1254 暴露还会扰乱卵母细胞和卵丘细胞之间缝隙连接介导的通讯。总之，猪卵母细胞在体外成熟过程中暴露于 PCB 会显着降低卵母细胞的发育能力，改变其细胞质重塑以及与体细胞的通讯。这些数据表明，多氯联苯在猪体内的积累可能对该物种的繁殖效率产生不利影响。
Polychlorinated biphenyls (PCBs) are one of the most persistent and widespread groups of endocrine disrupting compounds in the ecosystem. These substances are present in sewage sludge that is spread in increasing amounts on arable land and pasture as fertilizer, and are ingested by farm animals with food and drinking water. This study investigated the effect of different PCB concentrations on pig oocyte in vitro maturation and developmental competence as well as examined the possible mechanisms involved. A concentration ranging from 0 to 1 mg/mL of Aroclor 1254 (A1254), a pool of more than 60 PCB congeners, was added to the maturation medium, as its composition is considered environmentally relevant. A1254 had no effect on maturation of pig oocytes and on the number of oocytes that cleaved following parthenogenetic activation at any of the doses tested. By contrast, a significant decrease in the number of zygotes that developed to blastocyst stage became evident at a concentration of 10 ng/mL. The number of blastocysts obtained decreased significantly, and in a dose response manner with higher concentrations. Exposure to PCBs altered mitochondria relocation during maturation and this was associated with the lack of a cytoplasmic microtubule network. No effect on mitochondria activity was observed. A1254 exposure also perturbed gap-junction mediated communications between oocytes and cumulus cells. In conclusion, PCB exposure of pig oocytes during in vitro maturation significantly decreased oocyte developmental competence, altered both their cytoplasmic remodelling and the communication with the somatic compartment. These data indicated that accumulation of PCBs in the pig organism may have a detrimental effect on the reproductive efficiency in this species.