MMP28 (epilysin) as a novel promoter of invasion and metastasis in gastric cancer.
MMP28 (epilysin) as a novel promoter of invasion and metastasis in gastric cancer.
复制标题
MMP28（epilysin）作为胃癌侵袭和转移的新型启动子
DOI：
10.1186/1471-2407-11-200
复制
发表时间：
2011-05-26
期刊：
影响因子：
3.8
通讯作者：
Jian, Ni
中科院分区：
文献类型：
--
作者：
Jian, Pan;Yanfang, Tao;Jian, Ni
BackgroundThe purpose of this study was to investigate invasion and metastasis related genes in gastric cancer.MethodsThe transwell migration assay was used to select a highly invasive sub-line from minimally invasive parent gastric cancer cells, and gene expression was compared using a microarray.MMP28upregulation was confirmed using qRT-PCR. MMP28 immunohistochemistry was performed in normal and gastric cancer specimens. Invasiveness and tumor formation of stable cells overexpressing MMP28 were testedin vitroand in vivo.ResultsMMP28was overexpressed in the highly invasive sub-cell line. Immunohistochemistry revealed MMP28 expression was markedly increased in gastric carcinoma relative to normal epithelia, and was significantly associated with depth of tumor invasion, lymph node metastasis and poorer overall survival. Ectopic expression ofMMP28indicated MMP28 promoted tumor cell invasionin vitroand increased gastric carcinoma metastasisin vivo.ConclusionsThis study indicates MMP28 is frequently overexpressed during progression of gastric carcinoma, and contributes to tumor cell invasion and metastasis. MMP28 may be a novel therapeutic target for prevention and treatment of metastases in gastric cancer.
