The c-myc oncogene is translocated to the involved chromosome 12 in mouse plasmacytoma.
The c-myc oncogene is translocated to the involved chromosome 12 in mouse plasmacytoma.
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在小鼠浆细胞瘤中，c-myc 癌基因易位至相关的 12 号染色体。
DOI：
10.1073/pnas.82.12.4212
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发表时间：
1985
影响因子：
11.1
通讯作者：
Croce,CM
中科院分区：
文献类型：
--
作者：
Erikson,J;Miller,DA;Miller,OJ;Abcarian,PW;Skurla,RM;Mushinski,JF;Croce,CM
虽然已知c-myc癌基因在小鼠浆细胞瘤中与免疫球蛋白重链基因以头对头的方式重排，但尚不清楚c-myc癌基因是否易位到小鼠12号染色体上的重链位置，或者重链基因是否易位到小鼠15号染色体上的c-myc座位。为了确定这两种可能性中的哪一种是正确的，我们将中国仓鼠成纤维细胞与携带12号和15号染色体之间相互易位的小鼠浆细胞瘤细胞J558杂交，并检查了分离的杂交种是否存在正常和重排的小鼠c-myc基因。对于存在不同区域的小鼠重链基因，以及存在位于小鼠12号和15号染色体上的基因。本分析的结果表明，与人类Burkitt淋巴瘤的8；14号染色体易位，c-myc基因易位到小鼠浆细胞瘤的重链位点。因此，小鼠12号染色体上重链基因的定位和c-myc基因在15号染色体上的定位与人类同源基因的定位相同。
Although it is known that the c-myc oncogene is rearranged in a head-to-head fashion with the immunoglobulin heavy chain locus in mouse plasmacytomas, it has not been clear whether the c-myc oncogene is translocated to the heavy chain locus on mouse chromosome 12 or whether the heavy chain locus is translocated to the c-myc locus on mouse chromosome 15. To determine which of these two possibilities is correct, we hybridized Chinese hamster fibroblasts with J558 mouse plasmacytoma cells that carry a reciprocal chromosome translocation between chromosomes 12 and 15, and we examined the segregating hybrids for the presence of the normal and rearranged mouse c-myc genes, for the presence of different regions of the mouse heavy chain locus, and for the presence of genes located on mouse chromosomes 12 and 15. The results of this analysis indicate that, as in human Burkitt lymphomas with the 8;14 chromosome translocation, the c-myc gene is translocated to the heavy chain locus in mouse plasmacytomas. Thus the orientation of the heavy chain locus on mouse chromosome 12 and of the c-myc gene on mouse chromosome 15 is the same as the orientation of the homologous loci in man.