ISOLATION OF MONOCLONAL-ANTIBODIES TO PHENCYCLIDINE FROM ASCITES-FLUID BY PREPARATIVE ISOELECTRIC-FOCUSING IN THE ROTOFOR
ISOLATION OF MONOCLONAL-ANTIBODIES TO PHENCYCLIDINE FROM ASCITES-FLUID BY PREPARATIVE ISOELECTRIC-FOCUSING IN THE ROTOFOR
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DOI：
10.1016/0003-2697(88)90472-1
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发表时间：
1988-08-01
影响因子：
2.9
通讯作者：
BIER, M
中科院分区：
文献类型：
--
作者：
EGEN, NB;BLISS, M;BIER, M
一个单克隆抗体苯环利定的发展，产生在小鼠腹水，并纯化。在Rotofor和透析中，纯化仅使用双标度等电聚焦。在4h内，从10ml腹水中纯化25%（4mg）的抗体至均一，同时回收总抗体的63%。
A monoclonal antibody to phencyclidine was developed, produced in mouse ascites fluid, and purified. The purification used only preparative-scale isoelectric focusing in the Rotofor and dialysis. In 4 h, 25% (4 mg) of the antibody from 10 ml of ascites fluid was purified to homogeneity while 63% of the total antibody was recovered.