Pluripotent stem cells from the adult mouse inner ear
Pluripotent stem cells from the adult mouse inner ear
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DOI：
10.1038/nm925
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发表时间：
2003-10-01
期刊：
影响因子：
82.9
通讯作者：
Heller, S
中科院分区：
文献类型：
--
作者：
Li, HW;Liu, H;Heller, S
在哺乳动物中，获得性听力损失的永久性主要是由于耳蜗无法取代丢失的机械感受器细胞或毛细胞。相比之下，受损的前庭器官可以产生新的毛细胞，尽管数量有限。在这里，我们展示了成人的椭圆形感觉上皮含有显示干细胞特征的细胞。这些内耳干细胞具有自我更新的能力，并形成球体，表达发育中的内耳和神经系统的标记基因。内耳干细胞是多能的，在体外和体内可以分化为多种类型的细胞，包括代表外胚层、内胚层和中胚层的细胞。我们观察到这些干细胞能够分化为毛细胞样细胞，这意味着这种细胞可能用于替代丢失的内耳感觉细胞。
In mammals, the permanence of acquired hearing loss is mostly due to the incapacity of the cochlea to replace lost mechanoreceptor cells, or hair cells. In contrast, damaged vestibular organs can generate new hair cells, albeit in limited numbers. Here we show that the adult utricular sensory epithelium contains cells that display the characteristic features of stem cells. These inner ear stem cells have the capacity for self-renewal, and form spheres that express marker genes of the developing inner ear and the nervous system. Inner ear stem cells are pluripotent and can give rise to a variety of cell types in vitro and in vivo, including cells representative of ectodermal, endodermal and mesodermal lineages. Our observation that these stem cells are capable of differentiating into hair cell-like cells implies a possible use of such cells for the replacement of lost inner-ear sensory cells.