Immunosuppressive effect of an impurity of malathion: inhibition of murine T- and B-lymphocyte responses by O,O,S-trimethyl phosphorothioate.
Immunosuppressive effect of an impurity of malathion: inhibition of murine T- and B-lymphocyte responses by O,O,S-trimethyl phosphorothioate.
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马拉硫磷杂质的免疫抑制作用：O,O,S-三甲基硫代磷酸酯抑制小鼠 T 淋巴细胞和 B 淋巴细胞反应。
DOI：
10.1016/0041-008x(86)90228-0
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发表时间：
1986
影响因子：
3.8
通讯作者：
Imamura,T
中科院分区：
文献类型：
--
作者：
Thomas,IK;Imamura,T
体外研究了 O,O,S-三甲基硫代磷酸酯 (OOS-TMP) 对免疫反应的影响，例如抗原呈递、抗体产生和细胞毒性 T 淋巴细胞 (CTL) 功能。研究了 OOS-TMP 的非酶促和酶促谷胱甘肽 (GSH) 结合在这些反应中的作用。 (i) 用脾脏或 B 细胞直接培养； (ii) B细胞与T细胞在OOS-TMP与GSH强化胞质溶胶预孵育或不预孵育的情况下共培养。与未处理的对照相比，在这种处理后还评估了巨噬细胞的抗原呈递。 OOS-TMP 与 GSH 预孵育对细胞毒性 T 淋巴细胞和直接溶血斑形成细胞反应具有抑制作用。研究发现，这是通过对免疫系统的巨噬细胞、T 细胞和 B 细胞的直接抑制作用介导的，而不是通过调节性抑制 T 细胞的产生来介导的。因此，OOS-TMP在体外的抑制作用模式是由于抑制淋巴细胞增殖。这只有在谷胱甘肽存在的情况下才有可能，谷胱甘肽被确定为诱导 OOS-TMP 抑制作用的先决条件。
The effect of O,O,S-trimethyl phosphorothioate (OOS-TMP) on immune responsesssuch as antigen presentation, antibody production, and cytotoxic T-lymphocyte (CTL) function was examined in vitro. The roles of non-enzymatic and enzymatic glutathione (GSH) conjugation of OOS-TMP in these responses were studied. Antibody responses to T-dependent and T-independent antigens were evaluated after (i) direct culture with spleen or B cells; (ii) cocultivation of B cells with T cells with and without preincubation of OOS-TMP with GSH fortified cytosol. Antigen presentation by macrophages was also assessed after such treatment as compared to untreated controls. OOS-TMP preincubated with GSH had an inhibitory effect on the cytotoxic T lymphocyte and the direct hemolytic plaque forming cell responses. This was found to be mediated by a direct inhibitory effect on macrophages, T, and B cells of the immune system and not through the generation of regulatory suppressor T cells. Thus, the mode of suppressive action of OOS-TMP in vitro is due to inhibition of lymphocytic proliferation. This is only possible in the presence of glutathione which was determined to be a prerequisite for the induction of OOS-TMP suppressive effect.