Apomorphine does not alter amphetamine-induced dopamine release measured in striatal dialysates.
Apomorphine does not alter amphetamine-induced dopamine release measured in striatal dialysates.
复制标题
阿扑吗啡不会改变纹状体透析液中测量的安非他明诱导的多巴胺释放。
DOI：
10.1111/j.1471-4159.1990.tb01196.x
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发表时间：
1990
影响因子：
4.7
通讯作者：
Manley,LD
中科院分区：
文献类型：
--
作者：
Kuczenski,R;Segal,DS;Manley,LD
安非他明促进多巴胺从神经末梢的释放，但这种作用的机制尚未完全阐明。本实验旨在测试苯丙胺诱导的多巴胺释放在多大程度上依赖于多巴胺能神经元中的脉冲流和自身受体功能。用低剂量阿扑吗啡（0.05 mg/kg）预处理大鼠以抑制多巴胺神经元活性，并通过自由活动动物体内透析评估纹状体对苯丙胺（0.5 mg/kg）的多巴胺能反应。与以前的结果一致，阿扑吗啡单独大幅下降，而安非他明增加，纹状体透析液多巴胺浓度。然而，尽管阿扑吗啡预处理降低了对苯丙胺的运动反应，但苯丙胺诱导的透析液多巴胺增加不受影响。这些结果表明，苯丙胺促进多巴胺释放是独立的神经元放电和自身受体调节，与苯丙胺诱导的多巴胺释放的假定加速交换扩散机制一致。阿扑吗啡对安非他明运动激活的抑制作用的其他可能的机制进行了讨论。
Amphetamine facilitates the release of dopamine from nerve terminals, but the mechanisms underlying this effect have not been fully delineated. The present experiments were designed to test the extent to which amphetamine‐induced dopamine release is dependent on impulse flow and autoreceptor function in dopaminergic neurons. Rats were pretreated with a low dose of apomorphine (0.05 mg/kg) to inhibit dopamine neuronal activity, and the striatal dopaminergic response to amphetamine (0.5 mg/kg) was assessed by in vivo dialysis in freely moving animals. Consistent with previous results, apomorphine alone substantially decreased, whereas amphetamine increased, striatal dialysate dopamine concentrations. However, whereas apomorphine pretreatment decreased the locomotor response to amphetamine, the amphetamine‐induced increase in dialysate dopamine was unaffected. These results indicate that amphetamine‐facilitated dopamine release is independent of neuronal firing and autoreceptor regulation, consistent with the putative accelerative exchange‐diffusion mechanism of amphetamine‐induced dopamine release. Other possible mechanisms underlying the inhibitory effects of apomorphine on amphetamine locomotor activation are discussed.