Collagen Fibril Orientation in Tissue Specimens From Atherosclerotic Plaque Explored Using Small Angle X-Ray Scattering.
Collagen Fibril Orientation in Tissue Specimens From Atherosclerotic Plaque Explored Using Small Angle X-Ray Scattering.
复制标题
使用小角度 X 射线散射探索动脉粥样硬化斑块组织样本中胶原纤维的方向。
DOI：
10.1115/1.4052432
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Nelson,Drew
中科院分区：
文献类型：
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作者：
Silva,Herbert;Tassone,Christopher;Ross,ElsieGyang;Lee,JasonT;Zhou,Wei;Nelson,Drew
动脉粥样硬化斑块会在某些动脉中逐渐形成。斑块中纤维组织的破坏会导致斑块破裂和血栓栓塞，从而导致心脏病和中风。胶原原纤维是重要的组织构建块，组织强度取决于原纤维的方向。斑块组织中的原纤维取向可能会影响破坏的脆弱性。虽然 X 射线散射以前曾用于表征软组织和骨骼中的原纤维方向，但从未用于表征人类动脉粥样硬化斑块组织。本研究旨在利用小角 X 射线散射 (SAXS) 探索人类斑块样本中的原纤维取向。从人类股动脉和颈动脉中提取斑块组织，每个组织样本都含有钙化物质的区域。三维 (3D) 胶原原纤维方向是沿着扫描线确定的，扫描线从远离给定钙化点开始，然后向给定钙化点延伸。发现原纤维主要在远离钙化的大部分位置处沿斑块组织的圆周方向定向。然而，在许多情况下，钙化附近的主导原纤维方向不同，从圆周方向变为纵向或厚度（径向）方向。需要进一步的研究来阐明这些原纤维方向如何影响斑块组织的应力应变行为和破裂的脆弱性。
Atherosclerotic plaques can gradually develop in certain arteries. Disruption of fibrous tissue in plaques can result in plaque rupture and thromboembolism, leading to heart attacks and strokes. Collagen fibrils are important tissue building blocks and tissue strength depends on how fibrils are oriented. Fibril orientation in plaque tissue may potentially influence vulnerability to disruption. While X-ray scattering has previously been used to characterize fibril orientations in soft tissues and bones, it has never been used for characterization of human atherosclerotic plaque tissue. This study served to explore fibril orientation in specimens from human plaques using small angle X-ray scattering (SAXS). Plaque tissue was extracted from human femoral and carotid arteries, and each tissue specimen contained a region of calcified material. Three-dimensional (3D) collagen fibril orientation was determined along scan lines that started away from and then extended toward a given calcification. Fibrils were found to be oriented mainly in the circumferential direction of the plaque tissue at the majority of locations away from calcifications. However, in a number of cases, the dominant fibril direction differed near a calcification, changing from circumferential to longitudinal or thickness (radial) directions. Further study is needed to elucidate how these fibril orientations may influence plaque tissue stress–strain behavior and vulnerability to rupture.