Reduction of oligodendrocyte myelin glycoprotein expression following facial nerve transection
Reduction of oligodendrocyte myelin glycoprotein expression following facial nerve transection
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DOI：
10.1016/j.jchemneu.2008.08.006
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发表时间：
2008-12-01
影响因子：
2.8
通讯作者：
Tohyama, Masaya
中科院分区：
文献类型：
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作者：
Koyama, Yoshihisa;Fujiwara, Toshihiro;Tohyama, Masaya
少突胶质细胞髓鞘糖蛋白（OMgp）被认为在少突胶质细胞中表达，并通过与中枢神经系统（CNS）中神经元中表达的Nogo受体结合来抑制神经再生。然而，OMgp在CNS中的神经元以及少突胶质细胞中表达。为了帮助理解神经元OMgp的生理作用，我们检测了面神经切断后OMgp在面神经核中表达的变化。实时荧光定量PCR和Western blot分析显示，OMgp在面神经核中的表达在切断后5-7天（mRNA）或5-14天（蛋白质）呈小丑样调节。此后，OMgp的表达在轴突切断后28天恢复到对照水平。随后的分析，使用在筒仓杂交组织化学和免疫组化建立了OMgp表达的减少是由于在面部运动神经元的表达，但不是在少突胶质细胞。这些结果提示OMgp表达的改变可能参与了面神经元和上运动神经元之间神经回路的重建。(C)2008 Elsevier B. V.保留所有权利。
Oligodendrocyte myelin glycoprotein (OMgp) has been thought to be expressed in the oligodendrocytes and inhibit the regeneration of the nerves by binding to the Nogo receptor expressed in neurons in the central nervous system (CNS). However, OMgp is expressed in the CNS in the neurons as well as oligodendrocytes. In order to help understanding the physiological role of neuronal OMgp, we examined the change of OMgp expression in the facial nucleus after the facial nerve transection. Real-time RT-pCR and Western blot analysis showed a clown-regulation of OMgp expression in the facial nucleus 5-7 (mRNA) or 5-14 (protein) days after transection. Thereafter, expression of OMgp returned to the control level at 28 clays after axotomy. Subsequent analysis using in silo hybridization histochemistry and immunohistochemistry established that the decrease of OMgp expression was attributable to the expression in facial motoneurons, but not in oligodendrocytes. These findings suggest a possibility that the change of neuronal OMgp expression might be involved in reconnection of neural circuit between axotomized facial neuron and upper motor neuron after transection. (C) 2008 Elsevier B.V. All rights reserved.