Cytotactin expression in somites after dorsal neural tube and neural crest ablation in chicken embryos.
Cytotactin expression in somites after dorsal neural tube and neural crest ablation in chicken embryos.
复制标题
鸡胚胎背神经管和神经嵴消融后体节中细胞趋化素的表达。
DOI：
10.1073/pnas.88.15.6398
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发表时间：
1991
影响因子：
11.1
通讯作者：
Edelman,GM
中科院分区：
文献类型：
--
作者：
Tan,SS;Prieto,AL;Newgreen,DF;Crossin,KL;Edelman,GM
细胞外基质蛋白细胞趋化素/腱蛋白在体节发生过程中的时空表达表明，它在引起神经嵴（NC）发育模式的形态发生事件中发挥作用。在本研究中，利用原位杂交、蛋白质印迹和免疫组织化学检查了正常发育期间和损伤后体节中细胞趋化素的空间分布和分子形式。原位杂交表明，在 NC 细胞侵袭之前，细胞趋化素 mRNA 仅限于新形成的上皮体节的尾半部分。当每个上皮体节成熟时，产生骨节和皮肌节，mRNA首先被限制在皮肌节，随后限制在节骨部的头侧部分，这与之前报道的蛋白质分布一致。对切除了 NC 细胞的胚胎或保留 NC 细胞的简单伤口的胚胎中细胞趋化素和 NC 细胞分布的免疫细胞化学分析表明，NC 细胞的存在对于细胞趋化素的正确定位既不是必要的也不是充分的。免疫印迹分析表明，在不存在 NC 细胞的情况下，由造骨术合成的细胞趋化素与在存在 NC 细胞的情况下产生的细胞趋化素具有相似的分子质量。这些发现符合细胞趋化素分布异常与受伤过程相关的观点。我们的结论是，与斯特恩等人的建议相反。 [Stern, C. D., Norris, W. E., Bronner-Fraser, M., Carlson, G. J., Faissner, A., Keynes, R. J. & Schachner, M. (1989) Development 107, 309-319]，NC 细胞的存在与巩膜细胞趋化素的分布和分子质量之间不存在因果关系。
The spatiotemporal expression of the extracellular matrix protein cytotactin/tenascin during somitogenesis suggests that it plays a role in the morphogenetic events that give rise to the pattern of neural crest (NC) development. In the present study, the spatial distribution and molecular forms of cytotactin in somites were examined using in situ hybridization, Western blotting, and immunohistochemistry during normal development and after injury. In situ hybridization showed that prior to NC cell invasion cytotactin mRNA was restricted to the caudal half of the newly formed epithelial somites. As each epithelial somite matured, giving rise to a sclerotome and dermamyotome, the mRNA was first restricted to the dermamyotome and later restricted to the rostral protion of the sclerotome, consistent with the previously reported protein distribution. Immunocytochemical analysis of the distribution of cytotactin and NC cells in embryos with ablations that removed NC cells, or with simple wounds that left NC cells in place, demonstrated that the presence of NC cells is neither necessary nor sufficient for the correct positioning of cytotactin. Immunoblotting analysis showed that cytotactin synthesized by sclerotomes in the absence of NC cells was of similar molecular mass to that produced in their presence. These findings are in accord with the notion that the abnormalities of cytotactin distribution are related to the wounding process. We conclude that, contrary to the suggestion of Stern et al. [Stern, C. D., Norris, W. E., Bronner-Fraser, M., Carlson, G. J., Faissner, A., Keynes, R. J. & Schachner, M. (1989) Development 107, 309-319], there is no causal link between the presence of NC cells and the distribution and molecular mass of sclerotomal cytotactin.