Hibernation, a model of neuroprotection.
Hibernation, a model of neuroprotection.
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DOI：
10.1016/s0002-9440(10)64686-x
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发表时间：
2001-06
期刊：
影响因子：
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通讯作者：
F. Zhou;X. Zhu;R. Castellani;R. Stimmelmayr;George Perry;M. Smith;K. Drew
中科院分区：
文献类型：
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作者：
F. Zhou;X. Zhu;R. Castellani;R. Stimmelmayr;George Perry;M. Smith;K. Drew
冬眠是一种对脑缺血耐受的自然模型，代表了脑血流的明显波动状态，其中不发生脑损伤。许多神经保护方面可能有助于这种耐受性。本研究的目的是确定冬眠的脑组织是否耐受通过插入微透析探针模拟的穿透性脑损伤。在北极地松鼠开始冬眠之前，将引导套管手术植入北极地松鼠的纹状体。然后，在一些动物进入冬眠后，将微透析探针插入它们体内，而在另一些动物中，它们仍然保持体温。在植入微透析探针后3天，对冬眠和常温动物的脑组织进行了检查。苏木精和伊红染色的组织切片和免疫细胞化学鉴定的活化的小胶质细胞，星形胶质细胞和血红素加氧酶-1免疫反应性的检查表明，组织反应，显着衰减周围的探测轨道在冬眠动物相比，常温对照。未观察到组间导引插管周围组织反应的差异。对冬眠神经保护机制的进一步研究可能会为中风和创伤性脑损伤带来新的治疗策略。
Hibernation, a natural model of tolerance to cerebral ischemia, represents a state of pronounced fluctuation in cerebral blood flow where no brain damage occurs. Numerous neuroprotective aspects may contribute in concert to such tolerance. The purpose of this study was to determine whether hibernating brain tissue is tolerant to penetrating brain injury modeled by insertion of microdialysis probes. Guide cannulae were surgically implanted in striatum of Arctic ground squirrels before any of the animals began to hibernate. Microdialysis probes were then inserted in some animals after they entered hibernation and in others while they remained euthermic. The brain tissue from hibernating and euthermic animals was examined 3 days after implantation of microdialysis probes. Tissue response, indicated by examination of hematoxylin and eosin-stained tissue sections and immunocytochemical identification of activated microglia, astrocytes, and hemeoxygenase-1 immunoreactivity, was dramatically attenuated around probe tracks in hibernating animals compared to euthermic controls. No difference in tissue response around guide cannulae was observed between groups. Further study of the mechanisms underlying neuroprotective aspects of hibernation may lead to novel therapeutic strategies for stroke and traumatic brain injury.