Residual brain infection in relapsing-fever borreliosis
Residual brain infection in relapsing-fever borreliosis
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DOI：
10.1086/503367
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发表时间：
2006-05-15
影响因子：
6.4
通讯作者：
Lawson, CL
中科院分区：
文献类型：
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作者：
Cadavid, D;Sondey, M;Lawson, CL
背景神经系统受累在人类和实验动物的螺旋体感染回归热（RF）中很常见。RF最为人所知的是由2种大小的可变外膜脂蛋白（可变小（Vsp）和可变大（Vlp）蛋白）的顺序表达引起的抗原变异。不太了解的是RF疏螺旋体从血液中清除后在大脑中的持久性，称为残留脑感染（RBI）。我们的目标是研究RF中的RBI现象。我们研究了RBI的免疫功能正常的小鼠培养血液和脑灌流样品1个月后腹腔内接种疏螺旋体血清型1（Bt 1）。以Toll样受体2（TLR 2（-/-））或B和T细胞（scid）缺陷小鼠作为对照。所有scid小鼠血液和脑组织均有持续感染。在16只免疫活性和TLR 2（-/-）小鼠中的3只（19%）中发现RBI。RBI由原始血清型（Bt 1）或新出现的VSP（n = 1，更名为Bt3）或Vlp血清型的持续存在引起。Bt 1（Vsp 1）和Bt3（Vsp 3）的Vsp具有75%的一致性。RF中的RBI相对频繁，并且可以通过原始或新出现的血清型的持续存在而发生。
Background. Neurological involvement is common in the spirochetal infection relapsing fever ( RF) in both humans and experimental animals. RF is best known for antigenic variation caused by the sequential expression of variable outer membrane lipoproteins of 2 sizes, variable small ( Vsp) and variable large ( Vlp) proteins. Less understood is the persistence of RF borreliae in the brain after they are cleared from the blood, referred to as residual brain infection ( RBI). Our goal was to investigate the phenomenon of RBI in RF.Methods. We studied RBI in immunocompetent mice by culturing blood and perfused brain samples 1 month after intraperitoneal inoculation with Borrelia turicatae serotype 1 ( Bt1). Mice deficient in Toll-like receptor 2 ( TLR2(-/-)) or in B and T cells ( scid) were included for comparison.Results. All scid mice had persistent infection in blood and brain. RBI was found in 3 ( 19%) of 16 immunocompetent and TLR2(-/-) mice. RBI was caused by either persistence of the original serotype ( Bt1) or newly emerged Vsp (n = 1, renamed Bt3) or Vlp serotypes. The Vsp of Bt1 ( Vsp1) and Bt3 ( Vsp3) were 75% identical.Conclusions. RBI in RF is relatively frequent and can occur by persistence of the original or newly emerged serotypes.