Potato Aphid Salivary Proteome: Enhanced Salivation Using Resorcinol and Identification of Aphid Phosphoproteins
Potato Aphid Salivary Proteome: Enhanced Salivation Using Resorcinol and Identification of Aphid Phosphoproteins
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DOI：
10.1021/pr501128k
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发表时间：
2015-04-01
影响因子：
4.4
通讯作者：
Kaloshian, Isgouhi
中科院分区：
文献类型：
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作者：
Chaudhary, Ritu;Atamian, Hagop S.;Kaloshian, Isgouhi
蚜虫将唾液输送到植物中，并使用专门的口器或管心针获取植物汁液作为营养。蚜虫唾液具有重要的生物学意义，因为它含有调节宿主防御和代谢的效应子。虽然分析蚜虫唾液腺和识别分泌的蛋白质已被成功地使用，成功的蚜虫唾液的直接分析已受到限制，由于人工饲料中收集的唾液稀缺。在这里，我们提出了使用神经兴奋剂，间苯二酚，诱导蚜虫流涎。马铃薯蚜虫（Macrosiphum euphorandum）的唾液，保持在番茄上，收集在间苯二酚饮食。用质谱法鉴定唾液蛋白，并与现有的M。在水中收集的大戟唾液蛋白质组。还进行了比较分析与现有的唾液蛋白质组从其他蚜虫物种。在间苯二酚饮食中鉴定的大多数蛋白质也存在于水饮食中，并且代表除了大量未知之外具有过多功能的蛋白质。大约一半的唾液蛋白没有被预测为分泌或具有典型的分泌信号肽。我们还分析了M的磷酸化状态。鉴定了三种已知的蚜虫效应子Me_WB01635/Mp 1、Me 10/Mp58和Me 23，它们携带磷酸化标记。除昆虫蛋白外，还在蚜虫唾液中鉴定了番茄宿主蛋白。我们的研究结果表明，蚜虫唾液是复杂的，并提供了一个丰富的资源的功能特性的效应器。
Aphids deliver saliva into plants and acquire plant sap for their nourishment using a specialized mouthpart or stylets. Aphid saliva is of great importance because it contains effectors that are involved in modulating host defense and metabolism. Although profiling aphid salivary glands and identifying secreted proteins have been successfully used, success in direct profiling of aphid saliva have been limited due to scarcity of saliva collected in artificial diets. Here we present the use of a neurostimulant, resorcinol, for inducing aphid salivation. Saliva of potato aphids (Macrosiphum euphorbiae), maintained on tomato, was collected in resorcinol diet. Salivary proteins were identified using mass spectrometry and compared with the existing M. euphorbiae salivary proteome collected in water. Comparative analysis was also performed with existing salivary proteomes from additional aphid species. Most of the proteins identified in the resorcinol diet were also present in the water diet and represented proteins with a plethora of functions in addition to a large number of unknowns. About half of the salivary proteins were not predicted for secretion or had canonical secretion signal peptides. We also analyzed the phosphorylation states of M. euphorbiae salivary proteins and identified three known aphid effectors, Me_WB01635/Mp1, Me10/Mp58, and Me23 that carry phosphorylation marks. In addition to insect proteins, tomato host proteins were also identified in aphid saliva. Our results indicate that aphid saliva is complex and provides a rich resource for functional characterization of effectors.