Identification of three immediate-early genes of white spot syndrome virus
Identification of three immediate-early genes of white spot syndrome virus
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白斑综合症病毒三个立即早期基因的鉴定
DOI：
10.1007/s00705-011-1004-1
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发表时间：
2011-09-01
影响因子：
2.7
通讯作者：
Yang, Feng
中科院分区：
文献类型：
--
作者：
Lin, Fanyu;Huang, He;Yang, Feng
病毒即时早期（IE）基因通常编码对病毒复制至关重要的调节蛋白。它们的转录独立于从头病毒蛋白合成，直接由宿主转录因子驱动。本研究采用egfp报告基因法检测了锌指蛋白家族的白斑综合征病毒（WSSV） 12个预测调控基因在High Five细胞中的启动子活性。结果表明，wsv056、wsv403和wsv465三个基因启动子可以驱动报告基因的表达，RT-PCR分析显示，它们在感染wssv的小龙虾原代红细胞中的表达对蛋白质合成抑制剂环己亚胺（cycloheximide， CHX）不敏感。因此，它们是WSSV的IE基因。
Viral immediate-early (IE) genes generally encode regulatory proteins that are critical for viral replication. Their transcription, which is independent of de novo viral protein synthesis, is driven directly by host transcription factors. In this study, we examined promoter activities of 12 predicted regulatory genes of white spot syndrome virus (WSSV) belonging to the zinc finger protein family by EGFP-reporter assays in High Five cells. The results showed that the promoters of three genes (wsv056, wsv403 and wsv465) could drive reporter gene expression, and RT-PCR analysis revealed that their expression in WSSV-infected primary crayfish hemocytes was insensitive to the protein synthesis inhibitor cycloheximide (CHX). Therefore, they are IE genes of WSSV.