Determination of different recreational drugs in sweat by headspace solid-phase microextraction gas chromatography mass spectrometry (HS-SPME GC/MS): Application to drugged drivers
Determination of different recreational drugs in sweat by headspace solid-phase microextraction gas chromatography mass spectrometry (HS-SPME GC/MS): Application to drugged drivers
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DOI：
10.1016/j.jpba.2016.07.018
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发表时间：
2016-09-10
影响因子：
3.4
通讯作者：
Zaami, Simona
中科院分区：
文献类型：
--
作者：
Gentili, Stefano;Mortali, Claudia;Zaami, Simona
基于顶空固相微萃取（HS-SPME）和气相色谱/质谱（GC/MS）相结合的方法，开发了一种用于在路边控制中被拦截的驾驶员汗液中最常用的滥用药物的测定方法。采用DrugWipe 5A型汗液筛选装置，将专用的汗液垫在额头皮肤表面轻轻摩擦收集汗液。过程包括酸水解，HS-SPME提取滥用药物，但在碱性介质HS-SPME条件下直接提取Delta(9)-四氢大麻酚，毛细管柱气相色谱分离分析物，电子冲击电离质谱检测。该方法从定量限（LOO）到50 ng / pad呈线性（r(2) >= 0.99），测定内和测定间精密度和准确度始终小于15%，分析回收率在95.1%至102.8%之间，具体取决于所考虑的分析物。使用经过验证的方法，60名明显醉酒司机的汗液被发现对一种或多种滥用药物呈阳性，表明汗贴测试是一种可行的经济和简单的替代方法，可以替代对吸毒司机进行常规（血液和/或尿液）和非常规（口服液）滥用药物测试。(C) 2016 Elsevier B.V.版权所有
A procedure based on headspace solid-phase microextraction (HS-SPME) coupled with gas chromatography/mass spectrometry (GC/MS) has been developed for the determination of most commonly used drugs of abuse in sweat of drivers stopped during roadside controls.DrugWipe 5A sweat screening device was used to collect sweat by a specific pad rubbed gently over forehead skin surface.The procedure involved an acid hydrolysis, a HS-SPME extraction for drugs of abuse but Delta(9)-tetrahydrocannabinol, which was directly extracted in alkaline medium HS-SPME conditions, a GC separation of analytes by a capillary column and MS detection by electron impact ionisation. The method was linear from the limit of quantification (LOO) to 50 ng drug per pad (r(2) >= 0.99), with an intra- and inter-assay precision and accuracy always less than 15% and an analytical recovery between 95.1% and 102.8%, depending on the considered analyte.Using the validated method, sweat from 60 apparently intoxicated drivers were found positive to one or more drugs of abuse, showing sweat patches testing as a viable economic and simple alternative to conventional (blood and/or urine) and non conventional (oral fluid) testing of drugs of abuse in drugged drivers. (C) 2016 Elsevier B.V. All rights reserved.